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Relative ELISA Titer Analysis of RdRp Variants in Liver Cells

Comparison of relative ELISA titers (%) for various RdRp variants in liver cells. Results from Weng et al.'s study show differences in RdRp activity of HCR6, H77, JFH1, and other mutants. The polymerase activity and ethanol effects are also analyzed.

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Relative ELISA Titer Analysis of RdRp Variants in Liver Cells

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  1. Relative ELISA titer (%) Fig. S1. Weng et al.

  2. 1b 1a 2a HCR6 RdRp H77 RdRp JFH1RdRp GST-L245A/I253A A242C/S244D A238S/Q248E J6CF RdRpwt Protein marker RMT RdRpwt Con1 RdRpwt NN RdRpwt A242C S244D T251Q D244S L245A I253A wt wt wt kDa 200 116 97.2 66.4 44.3 29.0 20.1 Fig. S2. Weng et al.

  3. % 100 80 60 Relative polymerase activity 40 20 0 0 2 4 820 Ethanol (%) Fig. S3. Weng et al.

  4. 1b HCR6 2a JFH1 1a H77 wt L245A I253A D244S wt A242C/S244D wt A238S/Q248E + + - + + + + + - + + + + - - + - + + + - + + + + + - + + + + + - + + + + + - + + + + + - + + + + + - + + + RNA RdRp SM Fig. S4. Weng et al.

  5. H1 H2 A B Fig. S5. Weng et al.

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