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Unwinding RNA: CsdA Regulates RNase R Activity

Investigating how CsdA impacts RNase R function through RNA unwinding in E. coli. Time course study reveals modulation of RNase R with and without CsdA.

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Unwinding RNA: CsdA Regulates RNase R Activity

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  1. * 0.10 D272NRNase R 0.08 D280NRNase R D CsdA- C 0.06 Fraction unwound 0.04 0.02 0.00 0 5 10 15 20 25 Time (min) Supplemental Fig. S1 Awano et al A CsdA-DC RNase R-WT D272NRNase R D278NRNase R D280NRNase R Time (min) SS -P 0 1 3 5 10 15 20 0 1 3 5 10 15 20 0 1 3 5 10 15 20 SS -P 0 1 3 5 10 15 20 0 1 3 5 10 15 20 B

  2. 10bp * 3' 5' 5' 20 U+ 8-nt 3' 0.8 D CsdA C D272NRNase R 0.6 0.4 Fraction unwound 0.2 0.0 0 5 10 15 20 25 Time (min) Supplemental Fig. S2 Awano et al A CsdA-DC RNase R-WT D272NRNase R Time (min) ss 0 1 3 5 10 15 20 0 1 3 5 10 15 20 0 1 3 5 10 15 20 -P B

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