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2D-SDS Gel Electrophoresis. July 1, 2003 Theoretical basis for 2D-SDS Gel Elect The SWISS-2DPAGE Database Workshop. 2D-SDS Gel Electrophoresis What is it?.

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2d sds gel electrophoresis
2D-SDS Gel Electrophoresis
  • July 1, 2003
  • Theoretical basis for 2D-SDS Gel Elect
  • The SWISS-2DPAGE Database
  • Workshop
2d sds gel electrophoresis what is it
2D-SDS Gel ElectrophoresisWhat is it?
  • This is a method for the separation and identification of proteins in a sample by displacement in 2 dimensions oriented at right angles to one another.
  • This allows the sample to separate over a larger area, increasing the resolution of each component.
first dimension
First dimension
  • Separate by isoelectric point
  • Isoelectric point-pH at which the net charge on molecule is zero
visuals
Visuals

http://www.aber.ac.uk/parasitology/Proteome

/Tut_2D.html#Section%203

2nd dimension to separate by size
2nd Dimension-to separate by size
  • Five steps:
    • 1. Isolate proteins
    • 2. Denature proteins by adding sodium dodecyl sulfate
    • 3. Load onto gel
    • 4. Apply voltage gradient
    • 5. Stain or blot
slide7

http://www.davidson.edu/academic/biology

/courses/Molbio/SDSPAGE/SDSPAGE.html

slide8

http://www.davidson.edu/academic/biology

/courses/Molbio/SDSPAGE/SDSPAGE.html

slide9

http://www.davidson.edu/academic/biology

/courses/Molbio/SDSPAGE/SDSPAGE.html

slide15

http://www.weihenstephan.de/blm/deg/

manual/manualwork2html02testp9.htm

databases of 2d gels
Databases of 2D-Gels

http://www-lmmb.ncifcrf.gov/EP/table2Ddatabases.html

http://www.umh.ac.be/~biochim/balhf.htm