SDS-Polyacrylamide Gel Electrophoresis of Proteins. a.k.a. SDS-PAGE. NBD-chloride will bind to many of the same places ATP does… however it will also react with the protein and remain attached, unlike ATP.
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ATP does… however it will also react with the protein
and remain attached, unlike ATP.
Proteins that have been labeled somehow with a radioactive reagent will show up black when the gel is exposed to x-ray film.
Antibodies will detect the specific proteins they have been prepared against when incubated with protein blotted nitrocellulose.