1 / 14

Gel Electrophoresis

Gel Electrophoresis. Lab.8. Gel Electrophoresis. Gel Electrophoresis. Gel electrophoresis apparatus. An agarose gel is placed in this buffer-filled box and electrical field is applied via the power supply to the rear .

vera
Download Presentation

Gel Electrophoresis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Gel Electrophoresis Lab.8

  2. Gel Electrophoresis

  3. Gel Electrophoresis

  4. Gel electrophoresis apparatus An agarose gel is placed in this buffer-filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera.

  5. Agarosegel

  6. Polyacrylamide Gel

  7. Starch gel

  8. Gel conditions

  9. Gel conditions •  For instance, gels used in SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) will unfold and denature the native structure of a protein. In contrast to native gel electrophoresis, quaternary structure cannot be investigated using this method.

  10. Buffers

  11. Visualization

  12. Analysis

  13. DNA gel electrophoresis

More Related