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Nipponbare DNA

85H07S5. k3952. k2167. k4731. k3951. Nipponbare DNA. N1. N2. N3. N4. N5. N6. N7. N8. N9. N10. N11. N12. N13. N14. N15. N16. N17. N18. N19. 85H07S5. k3952. k3951. k2167. k4731. Tsuyuake DNA. T8. T10. T1. T2. T3. T4. T5. T6. T7. T9. T11. T12. T13. T14.

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Nipponbare DNA

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  1. 85H07S5 k3952 k2167 k4731 k3951 Nipponbare DNA N1 N2 N3 N4 N5 N6 N7 N8 N9 N10 N11 N12 N13 N14 N15 N16 N17 N18 N19 85H07S5 k3952 k3951 k2167 k4731 Tsuyuake DNA T8 T10 T1 T2 T3 T4 T5 T6 T7 T9 T11 T12 T13 T14 T15 T16 N1: inter-alpha-trypsin inhibitor heavy chain–2 precursor; N2: heat shock protein 70; N3: gag/poly protein; N4: neurofilament triplet H protein; N5: gag/poly protein; N6: NBS-LRR protein (Pikm1-NP); N7: NBS-LRR protein (Pikm2-NP); N8: copia polyprotein, N9: nucleolar protein Nop56; N10: NBS-LRR protein (Pikm3-NP); N11, C3H-1 protein; N12: NBS-LRR protein (Pikm4-NP); N13: unkonwn, N14: reverse transcriptase; N15, transposase, N16, NBS-LRR protein (Pikm5-NP), N17: NBS-LRR protein (Pikm6-NP), N18: tetratricopeptide repeat protein; N19: RNA helicase T1: NBS containing protein; T2: WRKY17; T3: inter-alpha-trypsin inhibitor heavy chain2 precursor; T4: heat shock protein 70; T5: WRKY transcription factor 41; T6: U-box domain containing protein; T7: profilaggrin;T8: reverse transcriptase; T9: RGH2B; T10: NBS containing protein; T11: inter-alpha-trypsin inhibitor; T12: zinc finger protein; T13: NBS-LRR protein (Pikm1-NP); T14: NBS-LRR protein (Pikm2-NP); T15: tetratricopeptide repeat protein; T16: RNA helicase Figure S1 Putative functions of genes predicted in the Pikm gene region.

  2. Figure S2 Multiple DNA sequence alignmentof Pikm1-TS and Pikm2-TS(page 1) Pikm1-TS ATGGAGGCGGCTGCCATGGCCGTAACCGCAGCCACGGGGGCCTTGGCGCCCGTGCTAGTG Pikm2-TS ATGGAGTTGGTGG---TAGGTGCTTCCGAAGCCAC------CATGAAATCTCTCTTGGGC ****** ** * * * * *** ****** * ** * * * * Pikm1-TS AAGCTGGCCGCTTTGCTGGACGACGGGGAGTGCAATCTTCTGGAGGGGAGCCGGAGCGAC Pikm2-TS AAGCTGGGCAATCTTCTAGCCCA---GGAGTATGCTCTCATCAGCGGTATCCGTGGTGAC ******* * * * ** * * * ***** *** * ** * *** * *** Pikm1-TS GCAGAGTTCATCAGATCCGAGCTGGAGGCCGTGCACTCTCTCCTCACCCCAAATATCTTG Pikm2-TS ATCCAGTACATCAATGACGAGCTTGCCAGCATGCAGGCCTTCCTC---CGTGATCTCAGC *** ***** ****** * * **** * ***** * ** ** Pikm1-TS GGGAGGATGGGGGATGACGAT-GCGGCG--TGCAAGGATGGCTTGATTGCGGAGGTCCGG Pikm2-TS AACGTGCCAGAGGGTCACAGTCATGGCCACCGGATGAAGGACTGGATGAAGCAGATCCGA * * ** * ** * *** * * * * * ** *** * ** **** Pikm1-TS GAGCTGTCCTACGACCTGGATGATGCCGTCGACGACTTCTTGGAGCTCAATTTCGAGCAG Pikm2-TS GACATCGCCTATGATGTTGAGGACTGTATCGATGACTTT-----GCCCA----------- ** * **** ** * ** ** **** ***** ** ** Pikm1-TS CGAAGAAGCGCAAGCCCTTTCGGTGAGCTCAAGGCAAGAGTTGAGGAGCGTGTCTCCAAT Pikm2-TS ----------CCGCCTCCCTCAG-GATTCCA---TCAGCGATGCCAAATGGTCCTTC--- * * * ** * ** ** ** * ** * * ** * Pikm1-TS CGCTTCTCTGACTGGAAGCTACCGGCGGCGAGCCTTCCGCCGTCGTCGGTACACCGTCGA Pikm2-TS --CTACTC--ACAAAAATCTATGAACTATGGACATGGTGGCCACGTCGTGTGATTGCTT- ** *** ** ** *** * * * * * * ***** * * Pikm1-TS GCTGGCTTGCCGCCACCAGATGCAGGGCTGGTGGGGATGCACAAACGTAAGGAAGAGCTC Pikm2-TS -CCAACATTGCCCAACTCAAGGTACGGGCACAACAGAT-CGCAGATCGACGTAGTAGAT- * * * * * ** * * * ** *** * ** * * * * ** * Pikm1-TS ATCGAGTTGCTGGAACAAGGGAGCAGTGATGCTTCACGATGGCGCAAGCGAAAACCTCAT Pikm2-TS ACGGAGT-----GAACAACCCAGAA---------CACCTTGACAGTAGCAGCAGTGCCAG * **** ****** ** * *** ** * *** * ** Pikm1-TS GTCCCCCTCAGGATAATGGGAGGGGAAATGCAAAAAATCGTGTTCAAGATTCCCATGGTG Pikm2-TS GACCCGTGCTGTCAATTACGA----AATTGCTGAGTATCAGGTCACAAGCCCTCA----- * *** * * * * ** ** *** * *** ** * * ** Pikm1-TS GACGATAAGAGCCGTACAAAAGCAATGTCATTGGTTGCAAGTACGGTTGGAGTGCACTCG Pikm2-TS ---GATCATTGGTATAAAGGAGC-----CTGTGGGGATGAAGACGGTCATGGAGGA---G *** * * ** * *** * *** * ***** * * * * Pikm1-TS GTTGCAATCGCCGGTGACCTAAGAGACCAGGTTGTGGTGGTCGGTGATGGCATTGACTCC Pikm2-TS CTTGAGGT--TTGGTTAACTAATCCTCAAGCTGAAAATGGGCAA----GCTGTTCTGTCC *** * *** * **** * ** * *** * * ** *** Pikm1-TS ATCAATCTGGTCTCTGCGCTCCGGAAGAAGGTGGGCCCTGCGATGTTTCTGGAGGTCAGC Pikm2-TS AT--AGTCGGTTTTGGAGGTGTGGGA-AAGACTACCATTGCCA-------------CAGC ** * *** * * * * ** * *** * *** * **** Pikm1-TS CAAGTAAAGGAAGATGTGAAGGAGATAACGGCGATGCTTGCGCCGGTGAAATCCATATGT Pikm2-TS ATTGTACAGAAAAGTCAGTGAAAAATTTCAGTGCCG--GGCATCAGT---AGCTGTGTCT *** ** ** * * * ** * * * * ** * ** * * * * * Pikm1-TS GAATTTCACGAGGTCAAAACAATTTGCATCCTTGGATTGCCAGGGGGAGGCAA-AACAAC Pikm2-TS CAGAACTATGA--CCAAGGCAAAGTCC-TCAATAGTATTCTGAGTCAAGTCAGCAATCAG * * ** *** *** * * ** * * * * * ** ** ** * Pikm1-TS GATTGCCCGAGTACTATATCATGCATTGGGAACGCAGTTCCAATGCCGGGTTTTCGCATC Pikm2-TS GAGCAGGGCAGCAGCACAACAATTAGTGAGAAAAAGAACCTCACCTCAGGCGCTAAGAGC ** ** * * * ** * ** *** * * * ** * * * Pikm1-TS AATCTCTCCAAGTTCCAGCCCCAGTCCCAATCTAACAGAGACTCTTGCAGACATTTTCGC Pikm2-TS A---TGTTGAAGA--CAGCCCTGTCACTGCT----CAGAGGTAATTGTA--TATGTCAGC * * * *** ****** * * ***** *** * ** * ** Pikm1-TS TCAAGCACAACTAGGAGTAACTGATACACTTAGCACACCATATGGTGGGAGTGGGACCGG Pikm2-TS CAGAAAATGAT--GGAAACCCTGATA-ATACACCA-ATCAGGCTGCAGGA--AACAACGG * * * *** ****** * * ** * ** * *** * *** Pikm1-TS GAGAGCTCTTCAACAACATCTCATCGACAACATATCAGCTTTCCTCCTCAACAAAAAGTA Pikm2-TS ACGA-----TGATCAAAACCCCAG-AAAACTGGAACAGCT--CCTGGCCGAAAAGAGTTA ** * * *** * * ** * * * ***** *** * * ** * ** Pikm1-TS TCTCATTGTAATCGATGACATTTGGCATTGGGAAGAATGGGAAGTCATCAGAAAGTCCAT Pikm2-TS TATCCTCTTGATTGATGACATTTGGTCTGCCGAAACATGGGAGAGTATCAGATCGATTTT * ** * * ** ************ * *** ****** ****** * * Pikm1-TS TCCCAAGAATGATCTGGGTGGTAGAATAATCATGACTACTCGTCTTAATTCAATAGCTGA Pikm2-TS GCCTAAAAATAATAAAGGCGGTAGAATAATAGTGACTACAAGATTTCAAGCTGTTGGTTC ** ** *** ** ** *********** ******* * ** * * * * * Pikm1-TS GAAGTGCCACACTGATGACAATGATGTTTTTGTCTACGAAGTTGGGGATCTAGATAATAA Pikm2-TS AACATGCTCCCCTCTTGAAACTGAT-CGTTTGCATAC--AGTTGATTTTCTCACCGATGA * *** * ** *** * **** **** *** ***** *** ** * Pikm1-TS TGATGCTTGGTCGTTGTCTTGGGGGATAGCAACAAAGTCTGGGGCAGGCAACAGGATCGG Pikm2-TS CGAGTCCCAAAACTTATTCAATACAAGTATTTGTGAATCAAAGATAAGAAAAGATAGCAA ** * ** * * * ** * * * ** * * Pikm1-TS AACTGGAGAGGATAATTCATGCTATGATATTGTGAACATGTGTTATGGTATGCCTTTAGC Pikm2-TS CAAAGTAGACGAGCAAGTCCCTGAGGAAATATGGAAAATATGTGGGGGATTGCCTTTGGC * * *** ** * * ** ** *** ** *** ** ******* ** Pikm1-TS ACTTATTTGGCTGTCGTCAGCATTGGTTGGAGAGATAGAAGAATTAGGTGGTGCTGAAGT Pikm2-TS -CATAGTCAGC-ATGGCTGGTCTTGTCGCCTGCAACCCAAGGAAAGCCTGCTGC-GATTG * ** * ** * * * *** * * *** * ** *** ** Pikm1-TS GAAAAAATGTAGGGATTTGAGACACATAGAGGATGGTATTTTGGACATCCCATCCTTACA Pikm2-TS GAGTAAACTTTGCAAATCATTATTTCCAGAGCAAGAAACTCC-----TCTTACCCTCGAT ** *** * * * * * **** * * * * ** * *** NBS Region

  3. Figure S2 Multiple sequence alignmentof Pikm1-TS and Pikm2-TS(page 2) Pikm1-TS ACCATTGGCGGAGAGTTTATGCCTTGGTTATAACCATCTTCCTCTTTATCTGAGGACTTT Pikm2-TS GGTGTTA-CAAGGATACTGGATTGTTGTTACAATGATTTGCCTGCGGATCTGAAGACTTG ** * ** * * **** ** ** * *** ****** ***** Pikm1-TS GTTGTTGTACTGTAGTGCATACCATTGGTCTAACAGAATCGAAAGGGGTCGTCTGGTCAG Pikm2-TS CTTATTGTACTTGAGTATATTTCCGAAGGGTTGGAAAATTAGTAGGAAACGTTTGTCCCG ** ******* *** ** * * * * *** *** *** ** * * Pikm1-TS GAGGTGGATTGCGGAAGGATTTGT--GTCGGAAG--------------AGAAAGAA--GC Pikm2-TS GCGATGGATAGCTGAAGGTTTTGCTAATGAGAAGCAAGGGTTAACCCAGGAAAGAGTTGC * * ***** ** ***** **** * **** ****** ** Pikm1-TS AGAAGGTTACTTTGGCGAGCTTATTAACAGAGGATGGATTACGCAGCACGGAG-ACAACA Pikm2-TS AGAGGCATACTTTAATCAACTCAC-AAGAAGGAACTTAGTACGTCCCATGGAGCATGGCA *** * ****** * ** * ** * * * * **** ** **** * ** Pikm1-TS ACAGTTA-----TAATTAC-TATGAGATCCACCCCGTGATGCTGGCCTTCCTGAGATGCA Pikm2-TS GCAATGGGAAGGTAAAAACGTTTCAAGTTCATGACATGGTTCTTGAATACATCATGTCCA ** * *** ** * * * * ** * ** * ** * * * * * * ** Pikm1-TS AGTCCAAGGAGTACAATTTT-TTAACATGCTTGGGTCTGGGATCTGATACTAGTACTAGT Pikm2-TS AATCAATCGAAGAGAATTTTATTACTGTGGTTGG---TGGACACTGGCAG----ATGACT * ** * ** * ****** *** ** **** *** *** * * * * Pikm1-TS GCATCCTCCCCAAGGTTGATTCGCCGGCTGTCTCTTCAGGGGGG-GTATCCAGTGGACTG Pikm2-TS GCACCAAGC---AATAAAGTCCGTCGACTGTCGATGCAAAGCAGTGGATCCAATCG-TGG *** * * * * ** ** ***** * ** * * * ***** * * * Pikm1-TS CTTGTCAAGCATGAGTATGGATGTGTCACACACTTGCAGCCTTGTCGTCCTTGGTGACGT Pikm2-TS AAGTTCAACAAAAGGCCTGAACTTGGCTCAAGTGAGATCACTGACGGTGTTTGGGAACCT **** * * ** * ** * ** * ** ** **** ** * Pikm1-TS GGCGCGACCCAAGGGAATCCCCTTC-TATATGTTTAAGCGCTTGCGAGTGTTGGACCTTG Pikm2-TS GA----ACCATGTGCCATTCCATTCATTCAACTATGGGATAATACAGGTGCTGGATCTTG * *** * ** ** *** * * * * * * * *** **** **** Pikm1-TS AAGATAATAAGGATATACAGGATTCTCATCTGCAGGGCATATGTGAACAGTTAAGCCTCA Pikm2-TS AGGACTGGAAGGGTTTGAAAGAGAGACATATGACGGAGATATGTCAAATG------CTTT * ** **** * * * ** *** ** ** ****** ** * ** Pikm1-TS GAGTGAGGTACCTTGGTCTCAAGGGAACGCGGATCCGAAAGCTCCCTCAGGAGATGAGGA Pikm2-TS TACTCAAGTATTTGAGCATCCGACGAACAGAAATTTCCAAAATTCCCTCCAAGATTCAGA * * * *** * * ** **** ** ** * ** **** ** Pikm1-TS AGCTGAAGCATTTGGAGATTTTGTATGTGGGGAGCACTCGGATCAGTGAACTTCCGCAAG Pikm2-TS AACTTGAGTACTTGGAAACTCTTGACATAAGGGAGACATATGTCAGGGACCTGCCTAAGT * ** ** * ***** * * * * * ** ** **** ** ** ** * Pikm1-TS AGATTGGAGAGCTGAAGCATCTGCGGATTCTGGACGTGAGAAACACGGACATCACTGAGC Pikm2-TS CAATAGTCCAGCTAAAA------CGGATCATTAGCAT-----ACTTGGA-GGGAATAAAA ** * **** ** ***** * * * ** *** * * * Pikm1-TS TCCCACTGCAGATACGGGAGCTGCAGCATCTGCACACTCTGGACGTGAGGAACACTCCAA Pikm2-TS ACACACGGAAGGGGCTGAGGTTGC------------CTCAAGAAAAAAGTAAGAAGCCAA * *** * ** * * * *** *** ** ** ** * **** Pikm1-TS TCAGTGAGCTCCCGCCGCAGGTTGGCAAGCTGCAGAATCTCAAGATTATGTGCGTGAGGA Pikm2-TS TTAAAAACCCGTCGCCTCAAGG-----------AAAAACAAAGGAGCCCGCAAAGAAAGG * * * * **** ** * * ** * * ** * * * Pikm1-TS GCACTGGGGTTAGGGAGCTCCCAAAGGAGATTGGGGAGCTGAATCATCTACAGACTCTGG Pikm2-TS ATTCTTATCCCAAGAAAAAGGTAAAGGCGCAATGAAAGC-ACTCCGTGTACTG--TCAGG ** * * * ***** * * *** * * *** * ** ** Pikm1-TS ACGTGAGAAACACGAGGGTGAGAGAGCTGCCATGGCAAGCTGGCCAGATCTCCCAATCGT Pikm2-TS GATTGAGATTGTTGAGGAATCATCAGAAGTAGCTGCAGGCCTTCATCAGTTGACAGG-GC ***** **** ** * *** ** * * * ** * Pikm1-TS TGCGCGTGCTTGCCGGTGACAGTGGCGATGGCGTGCGGTTGCCCGAAGGCGTCTGCGAAG Pikm2-TS TAAGGAAGCTTGCCATATACAAGCTCAATATAACAAAGG---GTGGTGATACCTTCAAA- * * ******* *** * ** * * * ** * ** Pikm1-TS CTCTGATCAACGGTATTCCAGGGGCTACGCGTGCAAAATGCAGGGAGGTTCTGTCCATCG Pikm2-TS ---CAATTA-CAGTCCTCCATTGAGTAC-CTTG------GCAGCTGTGGTCTGCAGACTC ** * * ** **** * *** * ** **** * **** * Pikm1-TS CGATCATCGATCGTTTCGGACCTCCCCTTGTTGGGATATTCAAAGTTCCCGGCAGTCATA Pikm2-TS TGGCCATCAATGAT--GAGAATTCT--------GAATTTATCAACTCACTGGGCGACATG * **** ** * ** ** * ** * ** * * ** * *** Pikm1-TS TGCGTATCCCGAAGATGATCAAAGACCACTTCCGCGTTCTTTCTTGCCTAGACATCAGGC Pikm2-TS CCCGCGCCTCCAAGATATCTTG---TCGCCCTTGAG--CTGTCTGGC--AAGTTGGAGAA ** * * ***** * * * * ** *** ** * ** Pikm1-TS TCTGCCACAAGCTTGAGGATGATGACCAAAAGTTC-CTCGCCGAGATGCCCA-ACCTGCA Pikm2-TS GCTACC-CAAGT----GGATCACCAGCATCACTACTCTCAACAAGCTAACCATATCTGTA ** ** **** **** * * ** * * * *** * ** * *** * *** * Pikm1-TS GACGCTCGTGCTGAGGTTCGAGGCCCTACCAAGACAACCCATAACCATCAACGGCACAGG Pikm2-TS A----CAGTTCTTAGGACTGAAACTTTG--GAGATCCTCCACATT--TTACCTTCATTGT ** ** *** ** * * *** *** * * * * ** * Pikm1-TS CTTCCAGATGCTGGAGAGCTTCCGTGTCGACAGCCGGGTGCCAAGGATAGCCTTCCATGA Pikm2-TS TTTCCCTCACCTT---CGCCTTTTCACTTAGTGCAGCGAAGCAGGATCAGGACATAATAA **** ** ** * * ** * * ** * ** ** * Pikm1-TS AG-ACGCCATGCCCAACCTCAAGCTTCTCGAGTTCAAGTTCTACGCCGGCCCAGCAAGCA Pikm2-TS AGGACATCCTTGAGAATAATAAATTGGACAGTGATGGGGAAATCGTCATTCCAGCTG--A ** ** * * ** ** * * * ** * ***** * Pikm1-TS ACGATGCCATCGGCATCACCAACCTGAAGAGCCTCCAAAAGGTGGTCTTTCGGTGCTCGC Pikm2-TS AGGATTCAAGAGTCTTAAGCTGCTTCGCTT-CTTTGCACCTTTAGTGCCGAAGCTCAGCT * *** * * * * * * * * * * * * * ** * * Pikm1-TS CATGGTACAAGAGCGACGCCCCTGGCATCAGCGCCACCATTGACGTCGTGAAGAAAGAAG Pikm2-TS TTTTGGACAAGAATG-CAATGCCAGCACT--CGAAATCATTGAAATGCGGTTTAAAGACT * * ****** * * * *** ** * ****** * * ***** NBS Region LRR Repeats

  4. Figure S2 Multiple sequence alignmentof Pikm1-TS and Pikm2-TS(page 3) Pikm1-TS CCGAGGAGCATCCCAACCGGCCGATCACCCTCCTCATCAATGCTGGGTATAAGGAGATAT Pikm2-TS TCGAAGGTCTATTTGGC--ATCGAAATCCTTGAAAATCTCCGTGAGGTGCATCTCAAAGT *** * * * *** ** * *** * *** * * * Pikm1-TS CAAC--TGAGTCACACGGGAGCAGTGAAAACATTGCGGGCAGCAGTGGGATCGATACTGA Pikm2-TS TAGTGATGGGGCAGAAGCAATAACCAAGTTCCTTGTAAATGATTTGAAGGTTAATACTGA * ** * ** * * * * * * *** * * ******* Pikm1-TS GCCTGCACAGGCACAGCATGATAATCTCCCTGCTGTTCGAGATGACTACAAGGGAAAAGG Pikm2-TS GAAACCAAAAGTATTTGTTGATGGCATCGTCACTGCATGA-------------------- * ** * * * **** ** *** ** Pikm1-TS GATTCTTCTTGATGGCAGGTGTCCTACCTGCGGCCGAGCGACTAAAATTGAAGAGGAAAC Pikm2-TS ------------------------------------------------------------ Pikm1-TS CCAAGATCGAGTAGCAGATATTGAAATTCAAACAGAAACTACTAGCTAG Pikm2-TS -------------------------------------------------

  5. Pikm1-TS 28 DGECNLLEGSRSDAEFIRSELEAVHSLLTPNIL-GRMGDD-DAACKDGLIAEVRELSYDL 85 D +L E +RSDA FIRS+LEAVHSLL P I G G++ DA CKD L+AEVRELSYD+ Pikm5-NP 24 DEHLDLAERTRSDAMFIRSQLEAVHSLLLPRISWGMTGEEVDALCKDELMAEVRELSYDM 83 Pikm1-TS 86 DDAVDDFLELNFEQRRSASPFGELKARVEERVSNRFSD---WKLPAASLPPSSVHRRAGL 142 DDA+D+F PF ELK RVE+ VS RFSD W+ P PS Pikm5-NP 84 DDAIDEFFLEEPMAGGDGGPFDELKTRVED-VSKRFSDSRRWRPPVEQHQPSLTAATVDC 142 Pikm1-TS 143 PPPDAG----------LVGMHKRKE-ELIELLEQGSSD---ASRWRKRKPHVPLRIMGGE 188 PPP A LV M K+ E ELI+LLEQG+ ASRWR P + Pikm5-NP 143 PPPHARFVHNMMDVSELVEMDKQHEKELIKLLEQGADTSIYASRWRIATP------WHDK 196 Pikm1-TS 189 MQKIVFKIPMVD----DKSRTKAMSLVASTVGVHSVAIAGDLRDQVVVVGDGIDSINLVS 244 Q V K+P + D + + S + S ++ GD + + G+G + L+S Pikm5-NP 197 EQSTVVKVPEREWGFPDNRNSPFIWASDSFERLRSGSLCGDT---LRLDGEGANIRKLLS 253 Pikm1-TS 245 ALRKKVGPAMFLEVSQVKEDVKEITAMLAPVKSICEFHEVKTICILGLPGGGKTTIARVL 304 LR KVG A ++V ++ V+E T CEFHEVKTICILGLPG GKTT+A++L Pikm5-NP 254 TLRNKVGRAQLVQVEDKRKRVEEATKP-------CEFHEVKTICILGLPGAGKTTLAKLL 306 Pikm1-TS 305 YHALGT---QFQCRVFASISPSSSPSPNLTETLADIFAQAQLGVTDTLSTPYGGSGTGRA 361 Y T QFQ R F S+SP + NLT+TL DI Q +G + +TPY G+GT Pikm5-NP 307 YSHHSTTEQQFQHRAFVSLSPGA----NLTDTLTDILLQ--VGAYNDDATPYCGTGTPH- 359 Pikm1-TS 362 LQQHLIDNISAFLLNKKYLIVIDDIWHWEEWEVIRKSIPKNDLGGRIIMTTRLNSIAEKC 421 QQ+LIDNISA+L+ KKYLI+IDD+WHWEEWEVIRKSIPKNDLG RIIMTTRLNSIAEKC Pikm5-NP 360 -QQYLIDNISAYLIGKKYLIIIDDVWHWEEWEVIRKSIPKNDLGSRIIMTTRLNSIAEKC 418 Pikm1-TS 422 HTDDNDVFVYEVGDLDNNDAWSLSWGIATKSGAGNRIGTGEDNSCYDIVNMCYGMPLALI 481 DD D FVYE LD DAW L +A KS I N CYDIV+MCYGMPLALI Pikm5-NP 419 RNDDMDAFVYETEALDYVDAWLLCDKVARKSVTCMNI-----NPCYDIVDMCYGMPLALI 473 Pikm1-TS 482 WLSSALVGEIEELGGAEVKKCRDLRHIEDGILDIPSLQPLAESLCLGYNHLPLYLRTLLL 541 +SSAL EI+ L E + R LR +EDGILDIPSL+PLAESLCLGY+HLPLYLRTLLL Pikm5-NP 474 RVSSALAEEIQALDSDERQIWRALRRVEDGILDIPSLKPLAESLCLGYDHLPLYLRTLLL 533 Pikm1-TS 542 YCSAYHWSNR--IERGRLVRRWIAEGFVSEEKEAEGYFGELINRGWITQHGDNNSYNYYE 599 CS YHW + ++RGRLV RWIAEGFVSEEK AEGYF EL+ RGW+ G N YE Pikm5-NP 534 CCSVYHWLDGGIVQRGRLVTRWIAEGFVSEEKAAEGYFDELVGRGWMKHRG----LNEYE 589 Pikm1-TS 600 IHPVMLAFLRCKSKEYNFLTCLGLGSDTSTSAS----SPRL-IRRLSLQGGYPVDCLSSM 654 IHP+MLA LR KSKEYNF+TCLG GSDT TSAS SP + IRRL LQ GYP+ C SSM Pikm5-NP 590 IHPMMLAILRYKSKEYNFVTCLGTGSDTCTSASLSYSSPTMAIRRLCLQRGYPMKCFSSM 649 Pikm1-TS 655 SMDVSHTCSLVVLGDVARPKGIPFYMFKRLRVLDLEDNKDIQDSHLQGICEQL-SLRV-R 712 DVSHT SLV+LGDV G+P MFKRLRVLDLEDN I+DSHL+ ICEQL SLR+ + Pikm5-NP 650 --DVSHTRSLVILGDVI---GVPLDMFKRLRVLDLEDNIGIEDSHLKKICEQLESLRLLK 704 Pikm1-TS 713 YLGLKGTRIRKLPQEMRKLKHLEILYVGSTRISELPQEIGELKHLRILDVRNTDITELPL 772 YLGLKGTRI KLPQE++KLK LEILYV ST I ELP EIGELK LR LDVRNT I+ELP Pikm5-NP 705 YLGLKGTRITKLPQEIQKLKQLEILYVRSTGIEELPWEIGELKQLRTLDVRNTRISELPS 764 Pikm1-TS 773 QIRELQHLHTLDVRNT-PISELPPQVGKLQNLKIMCVRSTGVRELPKEIGELNHLQTLDV 831 QI EL+HL TLDV N ISELP Q+G+L++L+ + VR+T VRELP +IGEL HL+TLDV Pikm5-NP 765 QIGELKHLRTLDVSNMWNISELPSQIGELKHLQTLDVRNTSVRELPSQIGELKHLRTLDV 824 Pikm1-TS 83 RNTRVRELPWQAGQISQSLRVLAGDSGDGVRLPEGVCEALINGIPGATRAKCREVLSIAI 891 RNT VRELPWQAGQIS SL V DS +G+RLPEGVCE LI GIP A AKC EVLSI I Pikm5-NP 825 RNTGVRELPWQAGQISGSLHVHTDDSDEGMRLPEGVCEDLIKGIPKAELAKCSEVLSINI 884 Pikm1-TS 892 IDRFGPPLVGIFKVPGSHMRIPKMIKDHFRVLSCLDIRLCHKLEDDDQKFLA-EMPNLQT 950 +DR G P +GIFKV G H IPK+IKDHF VLS LDIR +KLE+DD +FLA MPNLQ Pikm5-NP 885 VDRLGSPPIGIFKVIGLHKSIPKLIKDHFNVLSSLDIRRYNKLEEDDHEFLANNMPNLQM 944 Pikm1-TS 951 LVLRFEALPRQPITINGTGFQMLESFRVDSRVPRIAFHEDAMPNLKLLEFKFYAGPASND 1010 LVLRFEA R+PI IN TGFQMLE F V+SRVPRI F E AMP LK LEFKFYAGP S D Pikm5-NP 945 LVLRFEAPQREPIIINRTGFQMLERFLVESRVPRITFQEGAMPKLKHLEFKFYAGPPSKD 1004 Pikm1-TS 1011 AIGITNLKSLQKVVFRCSPWYKSDAPGISATIDVVKKEAEEHPNRPITLLINAGYKEI-S 1069 IGIT+LKSLQKVVFRCS WYKSD PGI A IDVVKKEA +HPNRPI+LLI G KE+ + Pikm5-NP 1005 PIGITHLKSLQKVVFRCSKWYKSDNPGIKAAIDVVKKEARQHPNRPISLLITEGDKEVPN 1064 Pikm1-TS 1070 TESHGSSENIAGSSGIDTEPAQAQHDNLPAVRDDYKGKGILLDGRCPTCGR-ATKIEEET 1128 E+HGSSENI + A DD C +CGR +T I+E T Pikm5-NP 1065 IEAHGSSENIV---------------VVHAAPDDAIS--------CSSCGRTSTSIQEGT 1101 Pikm1-TS 1129 -QDRVADIEI 1137 +DR+ +++ Pikm5=TS 1102 VRDRIPAMDL 1111 nT motif Kinase 1a Kinase 2, Kinase 3a Figure S3 Alignment of the protein sequences of Pikm1-TS and Pikm5-NP. Because the sequences in both proteins are not similar around the N-terminal, the sequences around this region were not aligned.

  6. Pikm2-TS 1 MELVVGASEATMKSLLGKLGNLLAQEYALISGIRGDIQYINDELASMQAFLRDLSNVPEG 60 MEL VGASEATM+SLLGKLGNLLAQEY+L+SG+RGDIQYINDELASMQAFLRDLS V EG Pikm6-NP 1 MELAVGASEATMRSLLGKLGNLLAQEYSLVSGVRGDIQYINDELASMQAFLRDLSVVTEG 60 Pikm2-TS 61 HSHGHRMKDWMKQIRDIAYDVEDCIDDFAHRLPQDSISDAKWSFLLTKIYELWTWWPRRV 120 H+H +R KDWMKQIRD+AYDVEDCIDDFAHRLPQDSISDAK SF+LTK+YEL TWWPRR Pikm6-NP 61 HNHDNRRKDWMKQIRDVAYDVEDCIDDFAHRLPQDSISDAKCSFILTKMYELLTWWPRRD 120 Pikm2-TS 121 IASNIAQLKVRAQQIADRRSRYGVNNPEHLDSSSSARTRA--VNYEIAEYQVTSPQIIGI 178 IAS IA+LKVRAQQIADRR+RYGVNNPEH DSS+S R RA +IAEYQ T PQI+ I Pikm6-NP 121 IASRIAELKVRAQQIADRRNRYGVNNPEHCDSSNSPRPRAHAAAQDIAEYQDTKPQIVSI 180 Pikm2-TS 179 KEPVGMKTVMEELEVWLTNPQAENGQAVLSIVGFGGVGKTTIATALYRKVSEKFQCRASV 238 KEPVGMKTVME LE WLT PQ + G+AVLSIVGFGGVGKTTIA ALYRKVS KF C+ASV Pikm6-NP 181 KEPVGMKTVMENLEKWLTEPQPDKGRAVLSIVGFGGVGKTTIAMALYRKVSGKFDCQASV 240 Pikm2-TS 239 AVSQNYDQGKVLNSILSQVSNQE------------------QGSSTTISEKKNLT-SGAK 279 AVSQNYD+ +VL SIL+QVS QE QGSS+T S ++N SG K Pikm6-NP 241 AVSQNYDEDEVLRSILNQVSKQEEAGGSTESSSRDENTREPQGSSSTSSREENTAESGTK 300 Pikm2-TS 280 SMLKTALSLLRGNCICQPENDGNPDNTPIRLQETTDDDQNPRKLEQLLAEKSYILLIDDI 339 ML L + + GN D T +R QET Q +L++ LAEK YILLIDDI Pikm6-NP 301 RMLNKLKKALPLSLL-----GGNDDKTSVRQQETMGSLQLREELKRRLAEKRYILLIDDI 355 Pikm2-TS 340 WSAETWESIRSILP----KNNKGGRIIVTTRFQAVGSTCSPLET------------DRLH 383 WSA+TW SI I+P +N+K RIIVTTRF AVGSTCSP D LH Pikm6-NP 356 WSAKTWNSI--IIPFLPSENDKDSRIIVTTRFHAVGSTCSPRHKNDEATSSPGHGKDLLH 413 Pikm2-TS 384 TVDFLTDDESQNLFNTSICESKIRKDSNKVDEQVPEEIWKICGGLPLAIVSMAGLVACNP 443 VDFLT D+ +LFN SI + R D +K ++ KICGGLPLAIV+MAGLVACNP Pikm6-NP 414 KVDFLTGDKPLDLFNASIPDPMKRTDRDK-------KLSKICGGLPLAIVTMAGLVACNP 466 Pikm2-TS 444 RKACCDWSKLCKSLFPEQETPLTLDGVTRILDCCYNDLPADLKTCLLYLSIFPKGWKISR 503 KA DWSKLC+SLFP T L LDGVTRILDCCYNDLPADLKTCLLYLSIFPKGWKISR Pikm6-NP 467 NKANSDWSKLCESLFPYPVTTLNLDGVTRILDCCYNDLPADLKTCLLYLSIFPKGWKISR 526 Pikm2-TS 504 KRLSRRWIAEGFANEKQGLTQERVAEAYFNQLTRRNLVRPMEHGSNGKVKTFQVHDMVLE 563 KRL+RRWIAEGFA EKQGLT+E VAEAYFNQL RRNL+RP+EHGSNGKVK FQVHDMVLE Pikm6-NP 527 KRLARRWIAEGFATEKQGLTEEEVAEAYFNQLARRNLIRPVEHGSNGKVKAFQVHDMVLE 586 Pikm2-TS 564 YIMSKSIEENFITVVGGHWQMTAPSNKVRRLSMQSSGSNRGSSTKGLNLAQVRSLTVFGN 623 YIMSKSIEENFITVVGGHWQMTAPSNKVRRLS+QSSGS G+STKGLNLAQVRSLTVFGN Pikm6-NP 587 YIMSKSIEENFITVVGGHWQMTAPSNKVRRLSLQSSGSKHGNSTKGLNLAQVRSLTVFGN 646 Pikm2-TS 624 LNHVPFHSFNYGIIQVLDLEDWKGLKERHMTEICQMLLLKYLSIRRTEISKIPSKIQKLE 683 LNHVPFHSFNYGIIQVLDLE WKGLKERH+TEICQML+LKYLSIRRTEI+KIPSKI+KLE Pikm6-NP 647 LNHVPFHSFNYGIIQVLDLEGWKGLKERHVTEICQMLVLKYLSIRRTEIAKIPSKIEKLE 706 Pikm2-TS 684 YLETLDIRETYVRDLPKSIVQLKRIISILGGNKNTRKGLRLPQEKSKKPIKNPSPQGKTK 743 YLETLDIRETYV +LPKS+ QLKRI SILGGNKNTRKGLRLPQEK K +KNPSPQGKTK Pikm6-NP 707 YLETLDIRETYVEELPKSVGQLKRISSILGGNKNTRKGLRLPQEKRNKAMKNPSPQGKTK 766 Pikm2-TS 744 EPAKKGFLSQEKGKGAMKALRVLSGIEIVEESSEVAAGLHQLTGLRKLAIYKLNITKGGD 803 EPA+KGFLSQEK KG MK+LRVLSGIEIV+ES+ VAA LHQLTGLRKLAIYKL I++ D Pikm6-NP 767 EPAEKGFLSQEKAKGTMKSLRVLSGIEIVDESAAVAASLHQLTGLRKLAIYKLKISEEND 826 Pikm2-TS 804 TFKQLQSSIEYLGSCGLQTLAINDENSEFINSLGDMPAPPRYLVALELSGKLEKLPKWIT 863 TFK+L SSIEYLGSCGLQTLAINDENS+FINSL +M APPRYLV+LELSGKL+ LP+WIT Pikm6-NP 827 TFKELLSSIEYLGSCGLQTLAINDENSKFINSLYNMSAPPRYLVSLELSGKLKWLPEWIT 886 Pikm2-TS 864 SITTLNKLTISVTVLRTETLEILHILPSLFSLTFAFSLSAAKQDQDIIKDILENNKLDSD 923 SITTLNKLTIS+TVL TETLEIL LPSLFSLTFAFSLSAAKQDQD +K ILE+NKL +D Pikm6-NP 887 SITTLNKLTISITVLTTETLEILRNLPSLFSLTFAFSLSAAKQDQDTVKGILEDNKLATD 946 Pikm2-TS 924 GEIVIPAEGFKSLKLLRFFAPLVPKLSFLDKNAMPALEIIEMRFKDFEGLFGIEILENLR 983 GEIVIPA+ FKSLKLLRFFAP VPKLSF DK+AMPALEIIEMRF++FEGLFGIEILENLR Pikm6-NP 947 GEIVIPAKEFKSLKLLRFFAPFVPKLSFPDKSAMPALEIIEMRFQEFEGLFGIEILENLR 1006 Pikm2-TS 984 EVHLKVSDGAEAITKFLVNDLKVNTEKPKVFVDGIVTA 1021 EVHLKVSDGAEAITKFLV+DLK NTEKPKVFVDGIVTA Pikm6-NP 1007 EVHLKVSDGAEAITKFLVSDLKDNTEKPKVFVDGIVTA 1044 nT motif Kinase 1a Kinase 2 Kinase 3a Figure S4 Alignment of the protein sequences of Pikm2-TS and Pikm6-NP

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