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This lab experiment examines the ability of lactase to break down lactose in milk into glucose and galactose. Four test tubes were prepared: one with a glucose solution, one with milk, one with lactase solution, and one combining milk and lactase. Using Benedict’s reagent, glucose presence was tested after heating. Results showed that milk with lactase produced a positive glucose test, confirming that lactase effectively breaks down lactose. This experiment highlights the important role of lactase in lactose digestion.
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DIGESTING LACTOSE WITH LACTASE Lab Conclusions
Part A Directions • Fill each clean test tube with 1 mL of the sample to be tested. Test tube 1: 1ml glucose solutionTest tube 2: 1ml milkTest tube 3: 1ml lactase solutionTest tube 4: 1ml milk plus 3 drops lactase solution (swirl gently to mix)
Part B Directions • Perform a Glucose test on each test tube using Benedict ’s reagent • Add 5-7 drops of Benedict’s Solution. • Heat test tube in a hot water bath for 3-5 minutes. • Check/Record color change. • A positive result will be orange.
Conclusions • Lactose in milk can be broken down into glucose and galactose. • We tested to see if the lactose in milk was broken down by detecting glucose • We used Benedicts’ reagent to detect glucose • The milk tested positive for glucose with lactase added in test tube 4 but was negative for glucose with out the lactase in test tube 2 • We can conclude that the added lactase caused lactose to be broken down because of the positive glucose test
