digesting lactose with lactase n.
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DIGESTING LACTOSE WITH LACTASE - PowerPoint PPT Presentation


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DIGESTING LACTOSE WITH LACTASE. Lab Conclusions. Background Information. Part A Directions. Fill each clean test tube with 1 mL of the sample to be tested.

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part a directions
Part A Directions
  • Fill each clean test tube with 1 mL of the sample to be tested.

Test tube 1: 1ml glucose solutionTest tube 2: 1ml milkTest tube 3: 1ml lactase solutionTest tube 4: 1ml milk plus 3 drops lactase solution (swirl gently to mix)

part b directions
Part B Directions
  • Perform a Glucose test on each test tube using Benedict ’s reagent
  • Add 5-7 drops of Benedict’s Solution.
  • Heat test tube in a hot water bath for 3-5 minutes.
  • Check/Record color change.
  • A positive result will be orange.
conclusions
Conclusions
  • Lactose in milk can be broken down into glucose and galactose.
  • We tested to see if the lactose in milk was broken down by detecting glucose
  • We used Benedicts’ reagent to detect glucose
  • The milk tested positive for glucose with lactase added in test tube 4 but was negative for glucose with out the lactase in test tube 2
  • We can conclude that the added lactase caused lactose to be broken down because of the positive glucose test