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Gel Electrophoresis

Gel Electrophoresis. Student Instructions Edvotek set up. Salk Institute Mobile Lab. Gel Electrophoresis Student Station Set-up. tip container w/tips. waste container. 5 µ l fixed pipettor. practice sheet in protector. foam epi rack w/ 1 food color tube each: orange, green, purple.

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Gel Electrophoresis

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  1. Gel Electrophoresis Student Instructions Edvotek set up Salk Institute Mobile Lab

  2. Gel Electrophoresis Student Station Set-up tip container w/tips waste container 5µl fixed pipettor practice sheet in protector foam epi rack w/ 1 food color tube each: orange, green, purple

  3. How to Use a 5µl fixed pipettor • Make sure a yellow tip is firmly twisted onto the end of the red pipettor • Carefully open one of the food color tubes • Don’t let the lid snap back and splash you! • Hold down the button on the top of the pipettor

  4. How to Use a 5µl fixed pipettor • While holding the button down, insert the tip into the food color tube

  5. How to Use a 5µl fixed pipettor • Release the button and remove from the food color tube • The tip now contains just 5 microliters of solution!

  6. How to Use a 5µl fixed pipettor • To dispense the solution push down on the button again • Using your other hand to guide the end of the tip, practice dispensing the 5µl drops • Try to not push down on the plastic sheet - see if you can hold the pipettor tip just above the paper

  7. How to Use a 5µl fixed pipettor • Twist off the dirty tip into the waste container and replace with a clean tip • Always use a clean tip each time!!

  8. How to Use a 5µl fixed pipettor • Ready to load a real gel?

  9. How to Use a 5µl fixed pipettor • Load the food color solutions into the wells on your group’s gel • Be careful not to poke through the bottom of the wells!

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