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  1. Welcome

  2. In Vitro Fertilization Hariom Yadav1, Shalini Jain1 and Mukesh Yadav2 1Animal Biochemistry Division, National Dairy Research Institute, Karnal-132001, Haryana, INDIA 2SOS in Chemistry, Jiwaji University, Gwalior-474011, M.P., INDIA Corresponding author: Email:

  3. Female reproductive system

  4. Male reproductive system

  5. Normal Fertilization

  6. IVF: In vitro fertilization In vitro: in glass Fertilization: Ova + Sperm

  7. Basic Principle of IVF Hormonal treatment Female Harvest the ovum Mature Ova Keep to develop embryo Mix in a test tube Motile sperms Collect semen Transfer to mother Male Natural ejaculation

  8. Hormonal Treatments • Drugs currently in use include: • clomiphene citrate (Clomidâ, Seropheneâ) • human menopausal gonadotropin (hCG) • gonodotropin releasing hormone (GnRH) analog called leuprolide (Lupronâ) • Most of these drugs may be used alone or in a combination with others.

  9. Egg Harvest • Ultra Sound Guided Aspiration • Laproscopy

  10. Fig 1

  11. Oocytes with granulosa cells "Naked" Oocyte

  12. 8-cell embryo for transfer

  13. Blastocyst for transfer

  14. Implantation

  15. Fourteen Days after Initial Cell Division

  16. Viable Fetus

  17. After Birth

  18. Alternates of IVF • Gamete intrafallopian transfer (GIFT): GIFT is similar to IVF. It is used when a woman has at least one normal fallopian tube. Eggs are placed in this tube along with a man’s sperm to fertilize there. • Zygote intrafallopian transfer (ZIFT): ZIFT is tubal embryo transfer in which a woman’s eggs are taken from her ovaries, fertilized in the laboratory, and put back in the fallopian tubes rather than the uterus. • Assisted fertilization techniques when not enough sperm are available or sperm quality is not sufficient to fertilize include the following: • Partial zona dissection • Subzonal sperm injection • Intracytoplasmic sperm injection • Embryo cryopreservation (frozen fertilized egg and sperm)

  19. ICSI Stands for intracytoplasmic sperm injection. This process is used to inject a single sperm into each egg before the fertilized eggs are put back into the woman's body. The procedure may be used if the male has a low sperm count.

  20. Cryopreservation of Ova, Sperm and Embryo

  21. Risks Superovulation Stimulates Egg Development Ovarian Hyperstimulation Syndrome (OHSS)

  22. There may be a failure to recover an egg because:- follicles that contain mature eggs may not develop in the treatment cycle • - ovulation has occurred before time of egg recovery- one or more eggs cannot be recovered- pre-existing pelvic scarring and/or technical difficulties prevent safe egg recovery • The eggs that are recovered may not be normal; • There may be insufficient semen to attempt fertilization of the recovered eggs because the man is unable to produce a semen specimen, because the specimen contains an insufficient number of sperm to attempt fertilization, because the laboratory is unable to adequately process the specimen provided, or because the option to use a donor sperm as a "backup" was declined; • Fertilization of the eggs to form embryos may fail even when the egg(s) and sperm are normal; • The embryos may not develop normally or may not develop at all. Embryos that display any abnormal development will not be transferred; • Embryo transfer into the uterus may be difficult/impossible, or implantation(s) may not occur after transfer, or the embryo(s) may not grow or develop normally after implantation; • Any step in the IVF-ET process may be complicated by unforeseen events, such as hazardous or catastrophic weather, equipment failure, laboratory conditions, infection, human error and the like.

  23. Normal results Success rates vary widely between clinics and between physicians performing the procedure and implantation does not guarantee pregnancy. Therefore, the procedure may have to be repeated more than once to achieve pregnancy. However, success rates have improved in recent years, up from 20% in 1995 to 27% in 2001. Abnormal results An ectopic or multiple pregnancy may abort spontaneously or may require termination if the health of the mother is at risk. The number of multiple pregnancies has decreased in recent years as technical advances and professional guidelines have led to implanting of fewer embryos per attempt.

  24. Ethics • Bypassing the natural method of conception. • The creation of life in the laboratory. • Fertilization of more embryos than will be needed. • Discarding of excess embryos. • Unnatural environment for embryos. • Use of untested technology. • Not affordable for many. • Misallocation of medical resources. • Creation of embryos, then freezing them, and keeping them "in limbo". • Exposure of embryos to unnatural substances. • Destruction of embryos in research. • Potential to create embryos for medical purposes. • Potential to select embryos (PGD). • Potential to modify embryos. • Facilitation of the idea that embryos are commodities. • Financial rewards for IVF doctors dissuade them from recommending other methods to couples. • Infertility is treated as a disease and not as a symptom of underlying • medical problems.

  25. Separating the traditional mother-father model Pregnancy past menopause Religious objections

  26. Thank you