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PMX 500 – PGP expression

This study evaluates the expression of P-glycoprotein (PGP) in CCRF-CEM and Vincristine-resistant (VinR) cell lines using flow cytometry. The mean fluorescence intensity of PGP in CCRF-CEM VinR cells at a concentration of 1 mg/ml was noted as 15.3, contrasting with the 2.5 observed in non-resistant CCRF-CEM cells. Additional measurements in HT29 and WI38 cells showed mean fluorescence values of 1.6 and 2.2, respectively. Secondary antibody controls confirmed specificity, indicating significant PGP expression in resistant cells.

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PMX 500 – PGP expression

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  1. PGP expression assessed by flow cytometry. • CCRF-CEM and VinR (Vincristine resistant) cells, HT29 and WI38. CCRF-CEM VinR1mg/ml 20.4 Mean fluorescence = 11.5 CCRF-CEM VinR 1mg/ml 27.4 Mean fluorescence = 15.3 CCRF-CEM Mean fluorescence = 2.5 PMX 500 – PGP expression HT29 Mean fluorescence = 1.6 WI38 Mean fluorescence = 2.2 secondary antibody only PGP antibody + secondary antibody

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