Bac-to-Bac Baculovirus Expression System. Lab. of Molecular Genetics Ryu Hyun-Mi. Bac-to-Bac Baculovirus Expression System. An efficient site-specific transposition system to generate baculovirus for high-level expression of recombinant proteins
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Lab. of Molecular Genetics
PPH, MSC, SV40 PA, gentamycin resistance marker
1. baculovirus shuttle vector(bacmid) : mini-attTn7 target site, lacZα, kanamycin resistance marker
2. helper plasmid : transposase, tetracycline resistance marker
-high titer virus is produced from the initial transfection.
-lacZα gene(blue/white screening white colony : recombinant)