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Introduction to the Microscope. Care Parts Focusing Wet mounts Calculating magnification. Microscope Parts. Eyepiece. Body Tube. Revolving Nosepiece. Arm. Objective Lens. Stage. Stage Clips. Coarse Focus. Diaphragm. Fine Focus. Light. Base. Microscope Care.

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introduction to the microscope

Introduction to the Microscope

Care

Parts

Focusing

Wet mounts

Calculating magnification

slide2

Microscope Parts

Eyepiece

BodyTube

RevolvingNosepiece

Arm

ObjectiveLens

Stage

StageClips

CoarseFocus

Diaphragm

FineFocus

Light

Base

slide3

Microscope Care

  • Always carry with 2 hands- one hand on arm – other on base
  • Only use lens paper for cleaning
  • Do not force knobs
slide4

Using the Microscope

1/Use the coarse adj. to raise the body tube.

2/Turn the light on

3/Place slide on stage using stage clips

4/Position the lowest (shortest) objective into place

5/Without looking in the eyepiece, raise the stage to be very close to the objective but do not let them touch!

slide5

Using the Microscope

6/ Look through the eyepiece

7/Using the course adj., bring it into focus

7/ Use fine adj. to get into focus further (you may have to use the

fine adj. only if in using highest obj.)

8. Move slide around if need – still keeping it clipped

making a wet mount
Making a wet mount

1/Place specimen on slide

2/Place a drop of water on slide

3/Place cover slip on slide using the angle technique

4/Remove air bubbles by tapping gently with finger or pencil eraser

5/Dry excess water by taking a paper towel and dabbing the outside

to make a stained slide
To make a stained slide
  • 1/Make a wet mount with water and coverslip
  • 2/Place a drop of stain at one end of the cover slip
  • 3/ hold a piece of paper towel at the other end of cover slip- this will draw the stain across the speciman
calculating magnification
Calculating Magnification
  • Eyepiece = 10x
  • Scanning power obj = 4x
  • Low obj = 10x
  • Highest obj = 40x
  • Ex. – eyepiece x low =

4x x 10x = 40x total