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BSC 197: Molecular and Cellular Basis of Life Lab

BSC 197: Molecular and Cellular Basis of Life Lab. TA info. Drinie Clairardin sgclair@ilstu.edu Office/Lab: SLB 245/247 http://biology197.wikispaces.com/. Lab Info. Lab schedule can be found on the syllabus Course website: www.bio.ilstu.edu/bsc197

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BSC 197: Molecular and Cellular Basis of Life Lab

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  1. BSC 197: Molecular and Cellular Basis of Life Lab

  2. TA info • Drinie Clairardin • sgclair@ilstu.edu • Office/Lab: SLB 245/247 • http://biology197.wikispaces.com/

  3. Lab Info • Lab schedule can be found on the syllabus • Course website: www.bio.ilstu.edu/bsc197 • Lab will consist of an exercise one day and a wet lab the following day

  4. Lab Safety • Safety is the number one priority • ABSOLUTELY NO food, drink or tobacco in Lab • Lab safety equipment available and will be used as needed (eg. Goggles, gloves) • Location of Eyewash, fire extinguisher • Do not endanger yourself or others • Respect everyone in lab

  5. Assignments • All lab reports must be typed (including graphs) • Do the lab report as follows • Intro: Short background about the lab; include hypothesis • Materials and Methods: Summarize briefly; focus on the reasons, don’t need exact details • Results: tables/graphs of data and text to summarize what they show • Discussion: analysis of results; troubleshoot lab if it didn’t work • Conclusion: short summary of everything • All lab reports are due at the beginning of the lab, two days after the lab was completed

  6. Plagiarism Policy • Plagiarism will not be tolerated • Consequences of being caught for plagiarism include receiving a zero for the class and being referred to student affairs for disciplinary action • Do your own work • Make your own graphs • Write your own papers • Cite sources

  7. Exercise 1 • We will use several types of graphs in the lab • Bar (listed as column graph in excel): good choice when only using a few data points; can have words in the x-axis; x-axis is not evenly numbered

  8. Graphs (cont’d) • Line Graph: used when you are looking at a change over time; x-axis not drawn to scale and x-axis can have letters

  9. Graphs (cont’d) • Scatter (XY Scatter): X-axis drawn to scale; data will be plotted in its exact position not just at the labeled point; can not be used if there are letters needed on the x-axis

  10. Graphs (cont’d) • Pie chart: Used when describing a part or percentage of a whole

  11. Graphs (cont’d) • Graph requirements: • X and Y axis titles, labels and units • Graph title (must be descriptive) • Legend • Failure to include ALL requirements will result in point deductions

  12. Statistical Calculations • Pages 11-14 (top portion-not including T-Test) • Mean- Average • N = total number of replicates • X1, X2, X3… Individual data Points

  13. Mean Calculation Example • X1 = 79 • X2 = 85 • X3 = 92 • X4 = 87 • X5 = 93 • X6 = 99 • (79 + 85 + 92 + 87 + 93 + 99)=535 • 535/6=89.17

  14. Statistical Calculations (cont’d) • Standard Deviation (S.D.)- measures variability of the mean • N- number of replicates • (X2)- Sum of the squares of X • (X)2- The square of the sum of the values for X

  15. Standard Deviation (S.D.) Example Calculation Square X values N = 5 Sum of X = 0.583 Sum of X2 = 0.068599 S.D.= 0.012461942

  16. Statistical Calculations (cont’d) • Standard Error (S.E.): estimates reliability of the mean S.E.= 0.005573149 = 0.006 (rounded off)

  17. Statistical Calculations (cont’d) • Do not round values until the end • Show all your work for the calculations

  18. For tomorrow: • Exercise 1 due • Lab 1: The Properties of Enzymes • Read Lab 1 in the manual • Dress appropriately • No open toed shoes or shorts above the knee!

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