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Detection of microdeformation in agarose gel model of convection enhanced delivery

Detection of microdeformation in agarose gel model of convection enhanced delivery. By: Georgio Tsiagalis Mentor: Nikhil Sindhwani LPPD Director: Prof. Andreas A. Linninger 4/8/09. Background. Phenomena of reflux occurs during infusion into brain tissue.

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Detection of microdeformation in agarose gel model of convection enhanced delivery

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  1. Detection of microdeformation in agarose gel model of convection enhanced delivery By: Georgio Tsiagalis Mentor: Nikhil Sindhwani LPPD Director: Prof. Andreas A. Linninger 4/8/09

  2. Background • Phenomena of reflux occurs during infusion into brain tissue. • Deformation around the catheter opens a channel and fluid can flow into. • It has been established that there is detachment of the gel from the cannula shaft.

  3. Data collection for established results. • Triplicate experiments at various flow rate. • Experiment is not intended to measure the size of detachment, rather just to if the detachment is or not. Detachment Yes/No

  4. Trials 1 and 2 at 0.25μL/s (after 1 hour of infusion)Result: No detachment

  5. Trial 1 at 0.5μL/sResult: no detachment Trial 1 at 1 μL/s Result: Visible detachment

  6. Nanobeads and their uses • Nanobeads are black spheres 400nm in diameter. [Purchased from Phosphorex, Inc.] • Approaches: • Seeding beads in gel and infusing water. • Infusion of beads in gel.

  7. Experiments with Nanobeads (seeding) • Goal • To quantify the movement of beads seeded in the gel. • Materials • Solution of 400nm nanospheres in water. • Nanobead Seeding Method • “Nanobead-Gel Sandwich” technique • 1) a layer of gel is cast • 2) 1 drop bead solution is placed at catheter tip and left to dry. • 3) a layer of gel is cast on top

  8. Results • Experiment was a failure • Problems with current seeding technique • Gel to Gel interface does not merge during crystallization.

  9. Nanobead infusion Trial experiment 1 • Nanobead solution was infused into agarose gel at a freehand volumetric flow rate. • Results • Deformation occurs and nanobeads visibly flow in the formed channel.

  10. Trial Experiment 2 Before Infusion After 15 minutes Result: Failure due to pressure build-up that fractured the gel.

  11. Outline for final 3 weeks • Finish report by April 22 • This week - In progress • Deformation vs Flow Rate Experiments • Proving deformation occurs at volumetric flow rates of 1μL/s and 2μL/s, repetitive experiments. • Next week • Test the reversibility of detachment • Successfully show flow of beads in the deformation/channel

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