AMES TEST. By: Harjot Kaur Mentors: Dr. Mahesh Lakshman and Dr. Padmanav Pradhan Location: The City College of New York . ABSTRACT.
By: Harjot Kaur
Mentors: Dr. Mahesh Lakshman
and Dr. Padmanav Pradhan
Location: The City College of New York
The goal of my project is to evaluate the mutagenicity of the derivatives of common nucleosides. The derivatives of deoxy- and oxy-ribose nucleosides of adenosine and inosine are produced synthetically and purified by chromatography. Then we are going to test these derivatives for carcinogenic and mutagenic activities, by conducting an Ames Test. But before I started this test, I had to do trial Ames Test on control compounds: Adenosine, Inosine, Deoxyadenosine, and Deoxyinosine.
In the 1970s a scientist named, Bruce Ames, discovered a procedure that tests carcinogens in compounds. This procedure became to be known as the Ames Test. Studies show that carcinogens are easily detectable in microorganisms. Therefore, a bacterium Salmonella typhimurium was used. This organism cannot survive without the amino acid histidine.
In the procedure, the bacteria were given very little histidine in order to detect its mutation ability. This mutation ability tells us if the compound given to the bacteria is carcinogenic. If the Salmonella mutates, then the compound is carcinogenic. If the Salmonella does not mutates, then the compound is not carcinogenic.
7) Heat glass rod after dipping in alcohol.
8) Place agar plates onto rotating plate and spread
the bacteria using the heated glass rod.
9) Place 2 sterile paper discs onto the agar plates.
10) Label as desired: Control, A, I, DA, DI.
11) Place a couple of drops of the chemicals to its corresponding paper discs.
12) Rest for 30 minutes. Then incubate for 48 hours under 37ºC.
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