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WELCOME

WELCOME. EMERGING TRENDS IN ANTIMICROBIAL PEPTIDES. DEVELOPMENT AND APPLICATIONS. JITENDER MEHLA & Dr.R.A.SIDDIQUE RESEARCH SCHOLARS, ANIMAL BIOCHEMISTRY DIVISION N.D.R.I., Karnal, (Haryana) India. WHAT ARE AMPs?. PATHOGENS. HOST. INNATE IMMUNITY. DIFFERENT COMPONENTS.

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WELCOME

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  1. WELCOME

  2. EMERGING TRENDS IN ANTIMICROBIAL PEPTIDES DEVELOPMENT AND APPLICATIONS JITENDER MEHLA & Dr.R.A.SIDDIQUE RESEARCH SCHOLARS, ANIMAL BIOCHEMISTRY DIVISION N.D.R.I., Karnal, (Haryana) India

  3. WHAT ARE AMPs? PATHOGENS HOST INNATE IMMUNITY DIFFERENT COMPONENTS ANTIMICROBIAL PEPTIDES

  4. Cont… • Small peptides having app. 12-50 a. acids • Produced in most exposed tissues by induction or constitutively • Synthesized at low metabolic cost • So far more than 800 AMPs have been described

  5. Structure and Classification of AMPs Signal peptidase Elastase PRE PEPTIDE PRE PRO PEPTIDE 29-30 residues 12- 50 residues 98-114 residues

  6. Classification of AMPs

  7. Cont….

  8. Transcriptional regulation of AMPs Taeil Kim et.al., 2005

  9. Amino acid sequence of NK-Lysin

  10. KILRGVCKKIMRTFLRRISKDILTGKK Sequence of NK-2 Antimicrobial activity 1 5 10 15 20 25

  11. NK-CS Antimicrobial activity KLLRGVSKKIMRTFLRRISKDILTGKK

  12. NKCS-[MS] Antimicrobial activity KILRGVSKKISRTFLRRISKDILTGKK

  13. NKCS-shortened analogues KLLRGVSKKIMRTFLRRISKKDILTGKK

  14. NKCS- [20K] Antimicrobial activity KILRGVSKKIMRTFLRRISKK

  15. NKCS-[RKK] Antimicrobial activity RGVSKKIMRTFLRRISKK

  16. NKCS-[FR] Antimicrobial activity KILRGVSKKIMRTRLRRISKDILPGKK

  17. NKCS-[LP] Antimicrobial activity KILRGVSKKIMRTFPRRISKDILTGKK

  18. NKCS-[LP26] Antimicrobial activity KILRGVSKKIMRTPRRISKDILTGKK

  19. NK-2 NK-CS NKCS-[AA] NKCS-[FR] NKCS[MS] NKCS-[14] NKCS[15-27] NKCS-[17] NKCS-[LP] NKCS-[20K] NKCS-[LP26] NKCS-[RKK]

  20. Specifically targeted antimicrobial peptides(STAMPs) Repertoire of Antibiotics Microorganisms including normal flora Secondary infections CSP AMP CSP AMP Randal Eckertet. al., 2006 S.mutans

  21. Applications of AMP Innate immunity Fe-Metabolism Food preservation AMP Therapeutic potential Mitogens Signal transduction

  22. Therapeutic potential Zasloff et. al., 2002

  23. AMP and Fe metabolism

  24. Role of AMPs in Fe metabolism

  25. Food preservation Tali Rydlo et. al., 2006

  26. Difference in membrane contents High succeptibilty of tumor cells to AMPs Anti-tumor activity Anti-tumor activity Tumor cells Normal cells Wojciech Kamysz et. al., 2003

  27. High concentration Spectrum of biological activity of AMPs • Tumor cell lysis • Lysis of microbes • Stimulation of keratinocyte growth • Inhibition of protein kinase C • Stimulation of cytokine production • Stimulation of adheshion molecule expression Low concentration Wojciech Kamysz et. al., 2003

  28. CONCLUSION • AMPs are multifunctional peptides • These are having a wide spectrum of biological activities • Based on natural AMPs, synthetic analogs can be developed • These can be developed as Probiotic antibiotics

  29. THANKS

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