rotating vessel wall for the adhesion of cardiac cells
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Rotating Vessel Wall For the Adhesion of Cardiac Cells

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Rotating Vessel Wall For the Adhesion of Cardiac Cells. [Roli Kumar] [Advisor: Dr. Marc Lin]. Overview of Last Presentation. Importance of building a rotating vessel for replacement of damaged heart tissue and drug therapy research

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Presentation Transcript
overview of last presentation
Overview of Last Presentation
  • Importance of building a rotating vessel for replacement of damaged heart tissue and drug therapy research
  • objective was to develop 3-D cardiac tissue from a monolayer of cardiac cells
  • Basic design specifications of the rotating vessel
apparatus set up
Apparatus Set-Up

Suction cups

Plastic Rod

Plastic/metal

Rotating vessel

2.5cm

1.5cm

1.25cm

Magnetic Stirrer

how many cells are needed
How many cells are needed?
  • 1.6X 10^5 cells/cm^2
  • surface area= SA of cylinder+SA half circle
  • SA=2(pi)rh +.5(4(pi)r^2)
  • No. of cells= SA sphere X (cell/cm^2)
  • No. of cells= 3,455,200 cardiac cells
cardiomyocytes
Cardiomyocytes
  • Chick embryos
  • Chicks will be 7-8 days old
    • higher disassociation rate with younger chicks
    • older chicks produce low yield in disassociated cells
rotating vessel
Rotating Vessel
  • Metal ball
    • collagen will not bind
  • Plastic ball
    • collagen will bind
      • rat collagen
    • will not rotate with magnetic stirrer
      • aluminum foil
design specifications
Design Specifications
  • End-over end mixing
    • measurements at various speeds
    • problem: too fast, too slow
  • Solid body rotation
    • suspending cells in a liquid medium
    • inject the cells in
      • minimizing air bubbles
design specifications1
Design Specifications
  • Turbulence
    • suction cups
    • tubes connected
  • Aeration
    • medium usually contain bicarbonate
      • produces CO2
    • HEPES
      • not a bicarbonate buffer
work completed
Work Completed
  • Learned procedure for Cardiac Myocyte Isolation Protocol
    • cells were infected with bacteria
current work
Current Work
  • Repeat procedure using the ventricular portion of the heart
    • add penicillin to prevent bacterial growth
  • Sanitize rotating vessel
  • Put apparatus together
future plan of action
Future Plan of Action
  • Measure the speed of the rotating vessel
  • Model the forces applied on the cell
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