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Rotating Vessel Wall For the Adhesion of Cardiac Cells. [Roli Kumar] [Advisor: Dr. Marc Lin]. Overview of Last Presentation. Importance of building a rotating vessel for replacement of damaged heart tissue and drug therapy research

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Rotating Vessel Wall For the Adhesion of Cardiac Cells

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Rotating vessel wall for the adhesion of cardiac cells

Rotating Vessel Wall For the Adhesion of Cardiac Cells

[Roli Kumar]

[Advisor: Dr. Marc Lin]


Overview of last presentation

Overview of Last Presentation

  • Importance of building a rotating vessel for replacement of damaged heart tissue and drug therapy research

  • objective was to develop 3-D cardiac tissue from a monolayer of cardiac cells

  • Basic design specifications of the rotating vessel


Apparatus set up

Apparatus Set-Up

Suction cups

Plastic Rod

Plastic/metal

Rotating vessel

2.5cm

1.5cm

1.25cm

Magnetic Stirrer


How many cells are needed

How many cells are needed?

  • 1.6X 10^5 cells/cm^2

  • surface area= SA of cylinder+SA half circle

  • SA=2(pi)rh +.5(4(pi)r^2)

  • No. of cells= SA sphere X (cell/cm^2)

  • No. of cells= 3,455,200 cardiac cells


Cardiomyocytes

Cardiomyocytes

  • Chick embryos

  • Chicks will be 7-8 days old

    • higher disassociation rate with younger chicks

    • older chicks produce low yield in disassociated cells


Rotating vessel

Rotating Vessel

  • Metal ball

    • collagen will not bind

  • Plastic ball

    • collagen will bind

      • rat collagen

    • will not rotate with magnetic stirrer

      • aluminum foil


Design specifications

Design Specifications

  • End-over end mixing

    • measurements at various speeds

    • problem: too fast, too slow

  • Solid body rotation

    • suspending cells in a liquid medium

    • inject the cells in

      • minimizing air bubbles


Design specifications1

Design Specifications

  • Turbulence

    • suction cups

    • tubes connected

  • Aeration

    • medium usually contain bicarbonate

      • produces CO2

    • HEPES

      • not a bicarbonate buffer


Work completed

Work Completed

  • Learned procedure for Cardiac Myocyte Isolation Protocol

    • cells were infected with bacteria


Current work

Current Work

  • Repeat procedure using the ventricular portion of the heart

    • add penicillin to prevent bacterial growth

  • Sanitize rotating vessel

  • Put apparatus together


Future plan of action

Future Plan of Action

  • Measure the speed of the rotating vessel

  • Model the forces applied on the cell


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