1 3 development in imaging technology and staining techniques
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1.3 Development in imaging technology and staining techniques. Contrast. When light passes directly through cells in what is called brightfield microscopy, most cells are seen colourless

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Presentation Transcript
contrast
Contrast
  • When light passes directly through cells in what is called brightfield microscopy, most cells are seen colourless
  • Staining or using colouring agents showed that particular stains attach to particular parts of the cell, improving contrast between internal structures and producing better images
  • Can be as simple as adding methylene blue or iodine
  • Chemical preservatives “fix” the cell and allow for more complex staining procedure
    • This kills the cell so it is not possible to view living tissue
staining cells quick lab
Staining Cells- Quick Lab
  • Page 254
  • Do this in partners
resolution
Resolution
  • Is the ability to distinguish between two structures that are very close together
  • An image should have high resolution if it is to show its detail
  • The clarity of images depends on the resolving power of the microscope
    • However light microscopes efficiency is limited because as light focusses onto smaller and smaller diameters, the image becomes blurry
fluorescence microscopy
Fluorescence Microscopy
  • Read paragraph on page 256
  • https://www.youtube.com/watch?v=sCYX_XQgnSA
confocal technology
Confocal Technology
  • Read paragraphs on page 257-258
electron microscope
Electron Microscope
  • Uses beams of electrons instead of a light wave and is able to produce images that provide fine detail
  • The image is formed by the absorption or scattering of electrons because electron-dense materials do not let the electrons pass through
slide8
TEM
  • Transmission Electron Microscope depends on a beam of electrons passed through a very thin section of fixed (not alive) and stained tissue embedded in plastic
  • Can magnify up to 1 500 000x
  • A drawback is the difficulty in building up a three dimensional picture of a cell from a very thin section
    • There is great detail, but the area we are looking at is very small
light vs electron microscopes
Light vs Electron Microscopes
  • Copy down Table C1.1 on page 259
  • https://www.youtube.com/watch?v=WDkLxlQ3hBA
scanning electron microscope
Scanning Electron Microscope
  • This gives information about the surface features of a specimen.
  • Specimens are fixed and covered with an electron-dense material like gold which reflects electrons
  • 300 000x magnification
  • A form of the SEM has been developed to allow use to use live materials
electron micrographs
Electron Micrographs
  • Photographs taken by either the SEM of TEM
  • https://www.youtube.com/watch?v=SIOOLXbwWME
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