STAINS AND STAINING TECHNIQUES. BY SAMUEL AGUAZIM(MD). Why we should be Stain Bacteria : .
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Bacteria have nearly the same refractive index as water, therefore, when they are observed under a microscope they are opaque or nearly invisible to the naked eye.
Different types of staining methods are used to make the cells and their internal structures more visible under the light microscope.
Simple stains use one dye that stains the cell wall.
The cells are then visible against a light background.
Bacterial arrangement :
1. Crystal violet