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Investigation of TNKS1 and Plk1 Interaction in Cellular Functions

This supplementary figure showcases the interaction between TNKS1 and Plk1 in various cellular processes, including phosphorylation and cell cycle regulation. The experimental data provide insights into their roles in cell biology.

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Investigation of TNKS1 and Plk1 Interaction in Cellular Functions

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  1. SupplementaryFigure S1 a b Asyn Noco Asyn Noco DMSO DMSO XAV939 XAV939 - + - + PPase TNKS1 150 TNKS1 150 IP (αTNKS1) PAR IP (αTNKS1) PAR 150 150 PTTG1 Cyclin B1 Actin Actin

  2. SupplementaryFigure S2 shLuc shPlk1 pHA pHA-Plk1 Flag-TNKS1 Flag HA-Plk1 Endogenous Plk1 Plk1 Myc-TRF1 Myc RFP Actin

  3. SupplementaryFigure S3 a b shLuc shTNKS1 NuMA TNKS 1 TRF1 Plk1 Phospho-H3 Actin shLuc shTNKS1 c 15.7 Phosho-H3-positive cells (%) 3.9 shLuc shTNKS1

  4. Supplementary Figure S4 Plk1 TNKS1 DAPI Merge shLuc shTNKS1 Plk1 TNKS1 DAPI Merge shLuc shTNKS1

  5. SupplementaryFigure S5 HeLa MRC-5 L-132 WI-26 NCI-H1299 Calu-3 HCC-1833 HCC-2108 A549 HCC-1171 SK-MES-1 lung cells hyperphosphorylation TNKS1 PlK1 Actin

  6. SupplementaryFigure S6 a b His-TNKS1 GST-TRF1 GST-TRF1 - - + + GST GST-TRF1 Plk1 Input (1/5) Input (1/5) - - + + GST GST - - + + TNKS1 TRF1 Phospho-Ser + + + + + + - + - - + + TNKS1 Phospho-Ser TRF1 His-TNKS1 Plk1

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