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This study investigates the impact of different experimental conditions on DNA replication dynamics, presented through a series of supplementary figures (S1-S8). Key metrics, including replication percentages across various time points (20’, 60’) and conditions (EB and CTL), alongside specific replication proteins like MCM3, Cdc45, RPA, and PCNA, are detailed. Data reveals significant variations in DNA replication efficiency and reveals interactions between core replication factors under different treatments, aiding in understanding the molecular mechanisms governing DNA synthesis.
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Supplementary figure S2. A B DNA replication % DNA replication %
NU EB+NU EB ctl 20’ 60’ 20’ 60’ 20’ 60’ 20’ 60’ cdc45 RPA PCNA MCM3 Supplementary figure S3. A B DNA replication %
Supplementary figure S4. % DNA replicated
Supplementary figure S5. DNA replication %
Supplementary figure S6. - 5 20 60 ℗-MCM2 MCM7 Cdc45 RPA
Supplementary figure S8. Supplementary figure S7. EB 50µM CTL EB 90’ 30’ Ctl 90’ 10min 45min 10min 45min DNA DNA Lamin B3 DHCC