Cellular and Whole-Animal Respiration

1. Cellular and Whole-Animal Respiration GK-12 Workshop II Ashley Vorhees and Sarah Park Cesar E. Chavez High School

2. Cricket Respiration Lab Students will measure oxygen consumption in crickets at different temperatures using simple respirometers. Water level Pipette

3. Why teach this? • Integrates into cell bio curriculum

4. Why teach this? • Integrates into cell bio curriculum • Cell curriculum can be abstract and difficult to relate to

5. Why teach this? • Integrates into cell bio curriculum • Cell curriculum can be abstract and difficult to relate to • Students learn about cell functions in the context of whole-animal function

6. Why teach this?

7. Overview of Module • Pre-Lab Lecture Notes • Cellular respiration • Pre-Lab Worksheet • Effects of temperature on metabolism • Lab Experiment • Measuring oxygen consumption in crickets • Follow-up Questions • Reinforce understanding of lab • Extension Activity • Extrapolating human MR from cricket MR

8. I. Pre-Lab Notes

9. II. Pre-Lab Activity

10. III. Cricket Respiration Lab As cricket consumes O2, pressure inside chamber decreases O2 O2 Water level O2 KOH O2 O2 Pipette

11. III. Cricket Respiration Lab As cricket consumes O2, pressure inside chamber decreases O2 O2 Water level O2 KOH O2 Pipette CO2

12. III. Cricket Respiration Lab As cricket consumes O2, pressure inside chamber decreases O2 O2 Water level O2 KOH CO2 Pipette CO2

13. III. Cricket Respiration Lab As cricket consumes O2, pressure inside chamber decreases O2 O2 Water level CO2 KOH CO2 Pipette CO2

14. III. Cricket Respiration Lab As cricket consumes O2, pressure inside chamber decreases O2 Water level CO2 CO2 KOH CO2 Pipette CO2

15. III. Cricket Respiration Lab Amount of water that enters pipette is directly proportional to amount of O2 consumed by the cricket Measure volume difference and divide by minutes MR = mL O2 per minute O2 Water level CO2 CO2 KOH CO2 Pipette CO2

16. IV. Data Collection

17. IV. Data Collection • Graph the results • Should show linear increase in metabolic rate with temperature

18. V. Extension Activity

19. California Content Standards (7-8th)

20. California Content Standards (9-12th)

21. Prior Knowledge

22. Materials Timeline • Live crickets (large) • Small vials • Rubber stoppers • Graduated pipettes (≤2mL) • Cotton balls • 15% KOH solution • Water baths • Thermometers • Ice *Needs to be completed in 1 day

23. Assessment • Fill-in Notes • Pre-Lab Worksheet • Data Table • Comprehension Questions • Extension Activity

24. Today… • Work in your RS/CT pairs • 11:00-11:45 Set up and perform the cricket respiration experiment (Stations 1-4) • 11:45-12:00 Walk-through of Extension Activity

26. Extension Activity • Purpose • Help students see connection between module content and their own physiology • How • Estimate human metabolic rate based on cricket metabolic rate • Reinforces concepts: • Your cells use same cellular processes to make ATP as a cricket • Body size (# of cells) determines how quickly you use energy

27. What factors determine metabolic rate? • Temperature • Phylogeny • Body Mass Metabolic rate scales with body mass according to: MR = aM0.75 MR – metabolic rate M – body mass a – constant that depends on the animal in question

28. Scaling of metabolic rate Mass = 10 g Mass = 20 g MR = 10 mL O2/min MR = 16.8 L O2/min MR = aM0.75

29. How can we use this equation? • Two equations with and unknown. • Instead of having students solve both, we combine into one that uses a relative mass:

30. 200,000 0.02 200,000 9457 189 0.02 0.4 0.0004 Body Mass Temperature

31. Thank you!