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Cellular Respiration

Cellular Respiration. Cellular Respiration = Glucose Oxidation. Redox Reactions. Coenzyme NAD+ is an electron carrier NAD + - oxidized NADH + H + - reduced. NADH - Nicotinamide adenine dinucleotide. Coenzyme found in all cells Made of 2 nucleotides. Mitochondrial structure - label.

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Cellular Respiration

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  1. Cellular Respiration

  2. Cellular Respiration = Glucose Oxidation

  3. Redox Reactions

  4. Coenzyme NAD+ is an electron carrier NAD+ - oxidized NADH + H+ - reduced

  5. NADH -Nicotinamideadenine dinucleotide Coenzyme found in all cells Made of 2 nucleotides

  6. Mitochondrial structure - label

  7. Mitochondrial structure

  8. Cellular Respiration overview

  9. Totals entering oxidative phosphorylation: • 4 ATP from substrate phosphorylation • 10 NADH (2 from glycolysis) • 2 FADH2

  10. NADH from glycolysis  2 ATP (need 1 to shuttle NADH into mitochondria) • NADH  3 ATP • FADH2 2 ATP • This is theoretical yield • Total energy produced = 36 – 38 ATP molecules - 2 in glycolysis, 2 in Krebs, 32-34* in Oxidative phosphorylation • * 34 for plants (don’t spend an ATP to get NADH into mitochondria), 32 for animals

  11. Glycolysis • Glyco – glucose Lysis - splitting or breaking • Pg. 162-163 • How is glucose split?

  12. Glycolysis summary • How many reactions are required? • What catalyzes each reaction? • How many ATP are produced? • How many net ATP are produced? • What is the initial reactant? • What are the final products? • Where does this occur in the cell?

  13. Glycolysis

  14. Glycolysis summary • How many reactions are required? 10 • What catalyzes each reaction? Specific enzyme • How many ATP are produced? 4 • How many net ATP are produced? 2 • What is the initial reactant? glucose • What are the final products? Pyruvate, 2 ATP, 2 NADH • Where does this occur in the cell? cytosol

  15. Krebs cycle (aka citric acid cycle) • What is the starting molecules for the Krebs cycle? • What was the ending molecules of glycolysis?

  16. Krebs cycle (aka citric acid cycle) • What is the starting molecules for the Krebs cycle? Acetyl CoA • What was the ending molecules of glycolysis? • pyruvate

  17. Pyruvate to Acetyl CoA • Intermediate step: pyruvate oxidation • How many reactions needed to convert pyruvate to acetyl CoA? • What is “lost” in the process? • What is “gained” in the process? • Where does this occur?

  18. Pyruvate to Acetyl CoA • Intermediate step: pyruvate oxidation • How many reactions needed to convert pyruvate to acetyl CoA? 3 • What is “lost” in the process? CO2, electron to NAD+ • What is “gained” in the process? NADH, Acetyl CoA • Where does this occur? As pyruvate enters mitochondrion, in the mitochondrial matrix

  19. Krebs Cycle – 1st step • In first step: • Oxaloacetate (4 C) + Acetyl-CoA (2 C) yields citrate (6 C) • Oxaloacetate gets regenerated through Krebs cycle • “-ate” – conjugate bases of the organic acids • Carboxyl groups – can donate protons • i.e. citrate is the conjugate base of citric acid

  20. Krebs cycle – p. 165 • How many reactions? • What catalyzes these reactions? • How many ATP produced? • How are the ATP produced? • Where does the rest of the energy harvested go?

  21. Krebs Cycle

  22. Krebs cycle • How many reactions? 8 • What catalyzes these reactions? Specific enzymes • How many ATP produced? 1 per cycle (2 total) • How are the ATP produced? Substrate phosphorylation • Where does the rest of the energy harvested go? • Electron carriers: 3 NADH, 1 FADH2 per cycle • (6 NADH, 2FADH2 total)

  23. Krebs cycle • How many turns of the cycle for 1 molecule glucose? • What are the initial reactants? Final products? • Where does this occur?

  24. Krebs cycle • How many turns of the cycle for 1 molecule glucose? 2 – since glucose splits into 2 pyruvate • What are the initial reactants? Final products? • Initial: 2 Acetyl CoA, 6 NAD+, 2 FAD, 2 ADP • Final: 4 CO2, 6 NADH, 2FADH2, 2 ATP • Where does this occur? In the mitochondrial matrix

  25. Substrate level phosphorylation

  26. Substrate Level Phosphorylation • As each bond of glucose is broken, energy is released: • If enough energy released all at once, the energy is used to directly phosphorylate ADP to make ATP (substrate level phosphorylation)

  27. -- If amount of energy released is small, electrons are taken off as units of energy and handed to an electron shuttle, NADH NADH gathers all the electrons and passes them off to the electron transport chain , so they can make ATP through oxidative phosphorylation

  28. Oxidative Phosphorylation: Electron Transport Chain & Chemiosmosis • Electron Transport Chain – see diagram on handout • Where do the electrons for the electron transport chain come from? • Why are electrons transferred from carrier to carrier? • Why does FADH2 enter at a different point than NADH?

  29. Electron Transport Chain/Oxidative Phosphorylation • Electron Transport Chain – see diagram on handout • Where do the electrons for the electron transport chain come from? From glycolysis, intermediate, krebs • Why are electrons transferred from carrier to carrier? Transferred to more electronegative carrier • Why does FADH2 enter at a different point than NADH? Has higher electronegativity

  30. What atom is the final acceptor of the electron? • Why? • What does it form? • What is gained during this process?

  31. What atom is the final acceptor of the electron? oxygen • Why? Most electronegative • What does it form? water • What is gained during this process? A H+ gradient

  32. Oxidative phosphorylation • What is the purpose? • What is a chemiosmotic gradient? • How does this generate ATP?

  33. Oxidative phosphorylation • What is the purpose? To produce ATP from ADP • What is a chemiosmotic gradient? A difference in concentration of H+ ions across a membrane (can be used to do work) • How does this generate ATP? Flow of H+ ions through ATP synthase into mitochondrial matrix cause the ATP synthase to rotate- chemical energy converted to mechanical energy • This drives phosphorylation of ADP into ATP (ADP + inorganic phosphate)

  34. ATP Synthase Uses flow of hydrogen ions down gradient to form ATP from inorganic phosphate and ADP

  35. ATP synthase video • http://www.dnatube.com/video/104/ATP-synthase-structure-and-mechanism

  36. Cellular Respiration

  37. ATP Numbers . . . Not exact – Based on experimental data- 1 molecule glucose yields 29 ATP NADH – 2.5 ATP, FADH2 – 1.5 ATP NADH from glycolysis in cytosol – electrons get passed to NAD+ or FAD in mitochondrial matrix (which carrier makes a difference in total ATP) Also – some of the proton motive force powers mitochondrion’s uptake of pyruvate from cytosol, also transport of phosphate into mit. matrix

  38. Cellular respiration efficiency – about 40% of energy from glucose gets stored in ATP • The rest of the energy is lost as heat

  39. Thermoregulation • Reducing efficiency of cellular respiration • Hibernating mammals – need to maintain body temperature • Have a channel protein in inner mitochondrial membrane that allows protons to flow back down concentration gradient without generating ATP • Allows for oxidation of fats to generate heat without ATP production

  40. ATP without oxygen • If oxygen is not present, etc and oxidative phosphorylation can’t occur • 2 ways to produce ATP: • Anaerobic respiration – prokaryotic organisms in environment without oxygen • Use another final electron acceptor rather than oxygen, i.e. sulfur • Fermentation

  41. Fermentation • Makes ATP through glycolysis (only 2 ATP) • NADH transfers its electrons to pyruvate, so NAD+ can be used again in glycolysis • Alcoholic fermentation – pyruvate converted to ethyl alcohol and CO2 • Lactic acid fermentation – pyruvate converted to lactate

  42. What about prokaryotes? • Glycolysis – cytosol • Krebs cycle – cytosol • Electron transport chain – electron carriers in plasma membrane, gradient gets generated across plasma membrane • Do not need to transport electrons (in NADH) from glycolysis into mitochondria, so can get more ATP

  43. Evolution & Glycolysis • Glycolysis is widespread among organisms • Oldest fossils of bacteria 3.5 billion years old • O2 in atmosphere not until 2.7 billion years ago • Perhaps early cells got ATP just through glycolysis

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