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Spooling DNA. Source: Strawberries Octoploid (8 copies of their genomic DNA). DNA Extraction . Place strawberry in baggie and close baggie “mush” berry for about 2 min. Add DNA extraction buffer Close baggie, and “mush” berry again for 2 min. DNA Extraction.

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Spooling DNA


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spooling dna

Spooling DNA

Source: Strawberries

Octoploid (8 copies of their genomic DNA)

dna extraction
DNA Extraction
  • Place strawberry in baggie and close baggie
  • “mush” berry for about 2 min.
  • Add DNA extraction buffer
  • Close baggie, and “mush” berry again for 2 min.
dna extraction3
DNA Extraction
  • Set-up beaker with cheese cloth
  • Cut corner of baggie and pour strawberry solution into cheese cloth
  • Squeeze out solution into beaker, the waste/pulp is trapped in cheese cloth
  • The strawberry solution contains your DNA
dna extraction4
DNA Extraction
  • Fill the test tube 1/3rd to ½ with the strawberry solution.
  • Now very slowly using the pipet add ice cold ethanol down the side of the test tube. Add enough until the tube is about 3/4th filled and you should see a whitish material. You want two layers to form.
  • Now take the wooden stick and begin “spooling”. Place the stick in near the interface of ethanol and the strawberry solution and “twirl”.
  • DO NOT MIX ETHANOL WITH THE STRAWBERRY SOLUTION.