1 / 1

Laboratory Heat Survival Assay Method for Lethal Heat Treatment Evaluation

Implementation of a lethal heat survival assay to assess the viability of strains post heat treatment. Strains are cultured, treated at 50°C, and viability calculated post-incubation.

truda
Download Presentation

Laboratory Heat Survival Assay Method for Lethal Heat Treatment Evaluation

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Fig. S1 Lethal Heat Survival Assay Strains were grown to mid-logarithmic phase (5 x 106 cells per ml) in YEPD medium, washed in PBS, and concentrated to 2 x 108 cells per ml. Aliquots of 100 μI were prepared in Eppendorf tubes. Then cells were heated at 50°C in water bath for 60 minutes. Tubes were removed and immediately placed on ice. The aliquots were then diluted accordingly and plated onto YEPD plates. Colonies were counted after 3 days of incubation at 30°C, and the percent viability was calculated with respect to cells not heated.

More Related