Akt Signaling and Integrin Interaction in Cell Migration Study

Supplemental Figure 1 CA-Akt1 DN-Akt1 DN-Akt1 CA-Akt1 DN-Akt1 Control Control CA-Akt1 Control A B C (-) EGF; 12 Hr pS473Akt Migration (% of recovery) Vitronectin Fibronectin Laminin β-actin # # TR-C2D TR-C2 TR-C2N # # # # # * # # D E F Migration (% of recovery) SPARC/Osteonectin BSP/Osteopontin * # # #

Supplemental Figure 2 CA-Akt1 +WT β3 integrin DN-Akt1 +WT β3 integrin WT β3 integrin CA-Akt1 DN-Akt1 Control β3 integrin CA-Akt1 +DN β3 integrin B β-actin DN β3 integrin A CA-Akt1 Control β3 integrin β-actin

Supplemental Figure 3 B A Migration (% of recovery) Vitronectin #* ## ## ## CA-Akt1 Control DN-Akt1 CA-Akt 1 + WT β3 integrin DN-Akt1 + WT β3 integrin WT β3 integrin Migration (% of recovery) SPARC/Osteonectin C BSP/Osteopontin ** ## ## ## #* ## ## Control DN-Akt1 CA-Akt1 DN-Akt 1 + WT β3 integrin CA-Akt 1 + WT β3 integrin WT β3 integrin CA-Akt1 Control DN-Akt1 CA-Akt 1 + WT β3 integrin DN-Akt1 + WT β3 integrin WT β3 integrin

A B Supplemental Figure 4 Normalized Resistance # Control WT β3 integrin DN-Akt1 DN-Akt1 + WT β3 integrin Time (h) C D Normalized Resistance # # # Control WT β3 integrin CA-Akt1 CA-Akt1 + WT β3 integrin Time (h)

Akt Signaling and Integrin Interaction in Cell Migration Study

Akt Signaling and Integrin Interaction in Cell Migration Study

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