1 / 29

Fluorescent principle

Fluorescent principle. Fluorescent microscope. DAPI. FITC. Alexa 488. Tetramethylrhodamine/TRITC. Rhodamin. Rhodamin Red-X. Alexa 568. Alexa 647. Texas-Red and Texas-Red-X. Alexa 594. Cy-Series. GFP and GFPuv. E-GFP's and DsRed. Table of Fluorescent Proteins.

theola
Download Presentation

Fluorescent principle

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Fluorescent principle

  2. Fluorescent microscope

  3. DAPI

  4. FITC

  5. Alexa 488

  6. Tetramethylrhodamine/TRITC

  7. Rhodamin

  8. Rhodamin Red-X

  9. Alexa 568

  10. Alexa 647

  11. Texas-Red and Texas-Red-X

  12. Alexa 594

  13. Cy-Series

  14. GFP and GFPuv

  15. E-GFP's and DsRed

  16. Table of Fluorescent Proteins

  17. GFP is a 238-amino acid protein originally isolated from the bioluminescent jellyfish Aequoria victoria Can be expressed in any organism without additional cofactors What is green fluorescent protein (GFP) ?

  18. Structure of GFP • Structurally, GFP represents a new motif which has been named the “β-can” • The “can” consists of 11 β-strands and a central α-helix

  19. Fluorescence is mediated by a fluorophore located at the center of the protein GFP-Fluorophore

  20. Structure of the GFP-fluorophore • The fluorophore is derived from the tripeptide -Ser65-Tyr66-Gly67- • Formation of the fluorophore occurs in an autocatalytic manner

  21. Light sources for Fluorescence

  22. Filtersetsand GFP's

  23. Quantum efficiency of Chip

  24. New Filterset

  25. New Filterset-other flourochromes

  26. Old Filtersets

  27. Old filtersets- other flurochromes

  28. Antibodies used for fluorescence

  29. Laser and Fluorochromes

More Related