1 / 1

225565Supplemental_Figure_S4

1. 2. 3. 4. 5. 6. 7. 8. 9. 10.

Download Presentation

225565Supplemental_Figure_S4

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. 1 2 3 4 5 6 7 8 9 10 Supplemental Figure S4: Expression of the ZmISA1 transgene in the Arabidopsisisa1-1 isa2-2mutant host line. Leaf cell RNAswere extracted from the isa1-1 isa2-2host strain expressing ZmIsa1 using the plant RNeasy kit from Qiagen(Courtaboeuf, France). The RT-PCR amplification was performed using the OneStepRT-PCR kit from Qiagen(Courtaboeuf, France). Different amounts of the amplicons were loaded on a 1.5% agarose gel (lanes 2, 5 and 8: 2 µl, 5 µl and 20 µl respectively). The amplicons were then sequenced. To control that the amplification was not the result of DNA contamination, we did a second amplification with the same protocol, except that we avoid the reverse transcription step. Again, different amount of amplicons were loaded on a 1.5% agarose gel (lanes 3, 6 and 9 : 2 µl, 5 µl and 20 µl respectively). A molecular size marker was loaded on lanes 1, 4, 7 and 10 (Smart Ladder MW-1700-10 from Eurogentec, Seraing, Belgium).

More Related