Why Dextranase L ‘Amano ’? Technical presentation

1. Why Dextranase L ‘Amano’?Technical presentation Apr-13

2. 2 points to consider! • Powerful! (very high endo activity) -> Dextranase L ‘Amano’ works rapidly and efficiently on Dextran during the process -> Dextranase L ‘Amano’ dosage can be adjustable at the factory depending on the purpose • PURE! (very high purity) -> Dextranase L ‘Amano’ helps better crystallization

3. How powerful?? • Very quick Dextran hydrolysis • DP2 and DP3 production DextranaseL ‘Amano’

4. Tangible Advantages • Better crystalized sugar • Gain better yield • Rapid reduction of viscosity • Higher productivity in the capacity • Shorter process time

5. Other advantages • Easier maintenance of the equipment • Prevents clogging in the event of interruption of the process. • Energy saving • Lower content of Dextran in waste water

6. What we observed?Voices from the market… • Quality improvement! • Lower dextran content in the process -> less consumption of other chemicals -> lower quantity of waste water • Better particle distribution (medium size) of raw sugar • Better and solid crystal in raw sugar • Yield improvement • Raw sugar yield increase up to 5-7% • Save energy • Lower consumption of calcium carbonate • Lower quantity of waste water • Lower energy against raw sugar production / Mt • Trouble shooting! (to add Dextranase just before re-start of the process) • Longer life of filter cloth • Lower sugar loss

7. Crystallization Better crystal Irregular crystal Decrease of irregular crystals, increase of medium size crystals.

8. Dextranase 2ppm Ca(OH)22-4%less Preliming Cleaning Cutting Delivery DDS Diffusion juice Preliming juice 145→30ppm CO2 1st Carbonation 1st Carbon. juice Heat Filtr. Liming heating Cake Lower Turbidity, colour FP SO2 CO2 2nd Carbon. Filtr. 2nd Clear juice Sulfur. Filtr Sulfied juice Evap. 1st Clear juice Syrup Lower turbidity, colour Lower turbidity, colour 21→11ppm Final Molasses 1st Cryst. 1st Molas. 2nd Molas. 3rd Cryst. 3rd Sepr. 1st Sepr. 2nd Sepr. 2nd Cryst. 957→690ppm （water consump. 23% lower Lower energy consump. (1770→1651 GJ） Raw sugar Sugar loss 2.79→2.43% 122→52ppm Lower long crystal, mid size particle77→80%, lower colour 65→44

9. Lactate value Diffuser Glucose/Lactate analyzer 124ppm 239ppm Mingler Dextranase DDS Preliming 38ppm 137ppm ・We assume the infection of Leuconostoc during the process since the lactate value is increased. ・We are checking the relationship between the value and dextran amount

10. (Info) There are 2 types of infection Bacteria and fungi • Comes normally during the storage of raw material in hot condition, which leads to lower yield of sugar. • Normally a carbamate based microbiocide specially formulated to inhibit growth of bacteria and fungi in cane sugar mills. Sodium dithiocarbamate, Sodium diethydlthiocarbamate and alkyl dimethyl benzyl ammonium are used in some countries. Leuconostoc Mesnteroides • Under cold weather, Leuconostoc Mesnteroides makes dextran during the process, which causes filter problem and poor sugar quality (crystallization, fineness etc.) • An organic antimicrobial based on dual antibiotics with special ingredient effective for bacteriostatic and bactericidal action, Sodium dithiocarbamate is also used. • Through our experience in Japan, only Dextranase is used controlling MK value during the process (no anti-biotic agents at all).

11. Contact us! Amano Enzyme Europe Ltd. Swiss Branch WTC Lugano CH-6982 Agno +41 – 91- 604 6450 sugawara@amano.co.uk