Supplemental Figure 1. A B C. D E F. G H I.
A B C
D E F
G H I
Supplemental Figure 1 Effects of ABA, a chitin elicitor (CH), JA, OPDA, SA and H2O2 on GA-inducible PCD. Eight isolated aleurone layers were incubated either with 20 M ABA, 50 M CH, 200 M JA, 100 M OPDA, 200 M SA or 400 M H2O2 in the presence of 1 M GA3 in 1 mL of 20 mM CaCl2 for 36 hours, and visualized by FDA staining. (A) Mock, (B, C) GA3, (D) GA3+ABA, (E) GA3+CH, (F) GA3+JA, (G) GA3+OPDA, (H) GA3+SA and (I) GA3+ H2O2.