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Impact of ARF and c-Myc on Gene Expression in p53-/- and ARF-/- MEFs

This study investigates the relative activity and expression of various genes regulated by ARF and c-Myc in double knockout (DKO) MEFs lacking both ARF and p53. Using PDGFRβ and other promoter analyses, we assess the influence of ARF on gene regulation over time, particularly focusing on cell lines expressing the Rat1aMycER system. Key genes examined include cdk4, gadd45, and p15INK4b, with expression profiles measured at several time points post-treatment. This research elucidates the complexities of tumor suppressor pathways in cellular contexts.

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Impact of ARF and c-Myc on Gene Expression in p53-/- and ARF-/- MEFs

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  1. cul1 promoter ARF-/-MEFs p53-/-MEFs pdgfr promoter ARF-/-MEFs p53-/-MEFs relative activity relative activity c-Myc - + + - + + ARF - - + - - + c-Myc - + + - + + ARF - - + - - + ARF- c-Myc- ARF- c-Myc- OHT hrs 0 4 8 0 4 8 0 4 8 0 4 8 0 4 8 0 4 8 Rat1aMycER -ARF +ARF -ARF +ARF -ARF +ARF genes cad eIF4E gadd45 a b c relative expression OHT hrs 0 2 4 8 0 2 4 8 0 2 4 8 0 2 4 8 0 2 4 8 0 2 4 8 0 2 4 8 0 2 4 8 Cell line DKO p53-/- DKO p53-/- DKO p53-/- DKO p53-/- gene cdk4 cul1 gadd45 p15INK4b relative expression

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