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Graphene Oxide: Intrinsic Peroxidase Catalytic Activity and Its Application to Glucose Detection PowerPoint Presentation
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Graphene Oxide: Intrinsic Peroxidase Catalytic Activity and Its Application to Glucose Detection

Graphene Oxide: Intrinsic Peroxidase Catalytic Activity and Its Application to Glucose Detection

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Graphene Oxide: Intrinsic Peroxidase Catalytic Activity and Its Application to Glucose Detection

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  1. Graphene Oxide: Intrinsic Peroxidase Catalytic Activity and Its Application to Glucose Detection By Yujun Song, Konggang Qu, Chao Zhao, Jinsong Ren, and Xiaogang Qu*

  2. Introduction 1.GN的研究现状使其在医药诊断和生物技术方面的应用成为可能; 2.本文提出GO-COOH有类似过氧化物酶的作用,并简介研究成果。 1.提出过氧化物酶作为H2O2和葡萄糖诊断试剂的应用还有广阔的发展空间; 2.简介葡萄糖传感器的研究现状(特别强调了下HRP),并将本文中的GO-COOH和HRP进行了简单的对比。 Simple Cheap highly sensitive highly selective

  3. Catalytic mechanism

  4. Experimental Expandable graphite flake + NaCl ground 10min H2O2+GO+TMB H2O UV-vis filtration Glucose+GOx+GO+TMB Hummer’s method AFM FTIR EDX GO-COOH [1] [1] X. M. Sun, Z. Liu, K. Welsher, J. T. Robinson, A. Goodwin, S. Zaric, H. Dai, Nano Res. 2008, 1, 203.

  5. Results

  6. Results

  7. Results Ping-pong mechanism

  8. Results [LUMO] Electron transfer mechanism

  9. Results

  10. Results

  11. Conclusion • 1 GO-COOH possesses intrinsic peroxidase-like activity and its catalysis is strongly dependent on pH, temperature, and H2O2 concentration, similar to HRP. • 2 In the presence of H2O2 and peroxidase substrate TMB, GO-COOH can produce a blue color reaction. Kinetic analysis indicates that the catalysis follows a ping-pong mechanism, and GO-COOH has even higher catalytic activity to TMB than HRP. • 3 GO-COOH, as a mimic peroxidase, shows several advantages over natural enzymes, such as ease of preparation, low-cost, and stability. • 4 Compared to HRP and Fe3O4 nanoparticles, GO-COOH possesses high surface-to-volume ratios as well as high affinity to organic substrates through π-π and hydrophobic interactions. • 5 We design and develop a simple, cheap, and highly selective and sensitive colorimetric assay to detect glucose in buffer solution or diluted blood and fruit juice samples. Our work will facilitate the utilization of GO-COOH intrinsic peroxidase activity in medical diagnostics and biotechnology.

  12. Thank You !