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Analytical Characterization of miRNA Standard Curves and Urinary RNA Extraction Repeatability for qPCR Assay

This study presents supplemental figures showcasing the standard curves of five miRNAs using synthetic miRNA and the repeatability of urinary RNA extraction for hydrolysis probe-based RT-qPCR assays. Additionally, serum concentrations of 5 miRNAs in children with kidney diseases, including Henoch-Schönlein purpura nephropathy (HSPN), IgA nephropathy (IgAN), lupus nephritis (LN), and normal controls (NC), are analyzed. The results provide valuable insights into miRNA quantification and urinary RNA extraction reliability for diagnostic purposes.

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Analytical Characterization of miRNA Standard Curves and Urinary RNA Extraction Repeatability for qPCR Assay

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  1. Supplemental Fig. 1A. Standard curve of miR-30a-5p using synthetic miRNA.

  2. Supplemental Fig. 1B. Standard curve of miR-151-3p using synthetic miRNA.

  3. Supplemental Fig. 1C. Standard curve of miR-150 using synthetic miRNA.

  4. Supplemental Fig. 1D. Standard curve of miR-191 using synthetic miRNA.

  5. Supplemental Fig. 1E. Standard curve of miR-19b using synthetic miRNA.

  6. Supplemental Fig. 2. The repeatability of urinary RNA extraction for the analytical characterization of hydrolysis probe-based RT-qPCR assay.

  7. Supplemental Fig. 3. The serum concentrations of the selected 5 miRNAs in children with other kidney diseases. HSPN, Henoch-Schönlein purpura nephropathy, n = 18; Ig AN, IgA nephropathy, n = 15; LN, lupus nephritis, n = 11; NC, normal controls, n = 16.

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