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Genetic Analysis of A. nidulans Chromosomal DNA Digestion Patterns

Southern blot analyses were conducted on A. nidulans chromosomal DNA digested with various enzymes and hybridized with specific DNA fragments to understand genetic patterns.

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Genetic Analysis of A. nidulans Chromosomal DNA Digestion Patterns

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  1. B A C D E 6 6 wt SB3.5 wt SB2.3 wt SB3.5 SB2.7 wt SB2.7 wt ALF1.1 kb kb kb kb kb 7 4 4 7 7 3 kb kb kb kb kb Supplementary Fig. 1. Southern blot analyses. Chromosomal DNA of A. nidulans was digested with EcoRI. Abbreviations: wt, strain AXB4A2; SB3.5, alcAp-scpR overexpression strain; SB2.3, DinpB; SB2.7, DinpB,alcAp-scpR overexpression; ALF1.1, DafoA,alcAp-scpR overexpression. Blots (A) and (C) were hybridized with an scpR full length 834 bp DNA fragment obtained by PCR from A. nidulans chromosomal DNA with the oligonucleotides 3492 XmaI for and 3492 XmaI rev (Table 2). Blots (B) and (D) were hybridized with a 1 kb DNA fragment obtained by PCR from A. nidulans chromosomal DNA with the oligonucleotides 3496 up SspI for and 3496 up SspI rev (Table 2). Chromosomal DNA of A. nidulans of Blot (E) was digested with NdeI and hybridized with a 1.3 kb DNA fragment obtained by PCR from A. nidulans chromosomal DNA with the oligonucleotides 1029 up for and 1029 up rev (Table 2).

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