'Pcr' presentation slideshows

Pcr - PowerPoint PPT Presentation


Viral cultures

Viral cultures

Investigation strategies and methods. Viral cultures. May 2007. Learning objectives. At the end of the presentation, participants should: Understand the principle of cultivating viruses Understand the methods and problems with cultivating viruses. Techniques to identify viruses.

By libitha
(420 views)

Polymerase Chain Reaction

Polymerase Chain Reaction

Polymerase Chain Reaction. PCR. “PCR is one of those inventions like the internet, once you have used it, you cannot quite understand how people managed before it existed”. Nobel Prize 1993. Invented by Dr. Kary Mullis, Research Scientist, Perkin Elmer Cetus Corporation in April 1983.

By velika
(205 views)

Application of PCR-technique in biological labs

Application of PCR-technique in biological labs

Arsalaan naveed. Ayesha naeem. Bdar slam. Saba naseem. Uzair hashmi. Application of PCR-technique in biological labs. Applications of PCR in Diagnostics. Uzair hashmi. PCR for pathogen detection. other methods for detection Cultures and colony counting assay

By hien
(241 views)

qPCR

qPCR

qPCR. Taylor Britton. Introduction. Quantitative polymerase chain reaction ( qPCR ) Monitor the amplification of a targeted DNA molecule during PCR in real-time. Limitations of PCR quantification. Poor precision Low sensitivity.

By fraley
(495 views)

Real Time PCR Quantitative PCR

Real Time PCR Quantitative PCR

Real Time PCR Quantitative PCR. PCR: P olymerase C hain R eaction. … a method widely used in molecular biology to make many copies of a specific DNA segment. In general is an exponential amplification. # copies = 2 n.

By andrew
(491 views)

Today

Today

Today. Do you have PCR amplicons? Run gel *.ppt Background DNA and our PCRs Interpretation of PCR results What to do next?. Pour agarose gel now. Add 0.3 g agarose to 30 ml 1X TAE Microwave and pour as before (12 tooth comb. SNAIL AND PARASITES BIOLOGY. DNA. “identity, possibilities”

By andrew
(113 views)

Viveca Giongo Laboratório de Virologia Molecular, Departamento de Biologia Celular e Molecular/UFF

Viveca Giongo Laboratório de Virologia Molecular, Departamento de Biologia Celular e Molecular/UFF

Como a Reação em Cadeia da Polimerase (PCR), PCR em tempo real e o Sequenciamento de genes são ferramentas importantes na investigação científica e no diagnóstico laboratorial ?. Viveca Giongo Laboratório de Virologia Molecular, Departamento de Biologia Celular e Molecular/UFF.

By naiya
(167 views)

Real-Time PCR

Real-Time PCR

Real-Time PCR Real-time PCR monitors the fluorescence emitted during the reaction as an indicator of amplicon production at each PCR cycle (in real time) as opposed to the endpoint detection. Kvatitativní PCR v reálném čase

By lida
(230 views)

Introduction

Introduction

Risk of misdiagnosis due to allele dropout in molecular diagnostics: analysis of 30769 genotypes.

By chava
(51 views)

Spørsmål : Har han en infeksiøs bronkitt ? KOLS? Reaktiv postinfeksiøs bronkitt ? Anamnese :

Spørsmål : Har han en infeksiøs bronkitt ? KOLS? Reaktiv postinfeksiøs bronkitt ? Anamnese :

Kasuistikk - Luftveisinfeksjoner Lars Heggelund, S eksjon for infeksjonssykdommer , Medisinsk avdeling , Drammen sykehus , Vestre Viken HF. Spørsmål : Har han en infeksiøs bronkitt ? KOLS? Reaktiv postinfeksiøs bronkitt ? Anamnese : Tobakk Arbeidskarriere ? TBC eksponering ?

By avari
(204 views)

Genética Molecular em Análises Clinicas

Genética Molecular em Análises Clinicas

Genética Molecular em Análises Clinicas. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDOS NUCLEICOS. Técnicas de amplificação de ácidos nucleicos. PCR Real Time PCR NASBA/TMA. PCR. OPTIMIZAÇÃO DO PCR. [MgCl 2 ] Th [dNTP] [primers] [DNA] [inibidores]. Variações. RT-PCR Multiplex PCR Nested PCR.

By garron
(118 views)

Vivian, Tamiris e Poliana

Vivian, Tamiris e Poliana

Vivian, Tamiris e Poliana. PCR - DEFINIÇÃO. PCR. Polymerase. chain. reaction. Reação em cadeia de polimerase. PCR – BREVE HISTÓRICO. Descrito por Kary Mullis em 1983. Patenteado em 1989 pela Hoffman La Roche & Perkin-Elmer Corporation. Prêmio Nobel da Química  1930.

By lane
(90 views)

Inflamation and Mortality in HIV-infected Adults: Analisys of the FRAM Study Cohort

Inflamation and Mortality in HIV-infected Adults: Analisys of the FRAM Study Cohort

Inflamation and Mortality in HIV-infected Adults: Analisys of the FRAM Study Cohort Phyllis Tien, A Choi, A Zolopa, et al. 17th CROI, San Francisco 2010. Abstract 725. Objetivo y Métodos:. Abstract 725.

By kolina
(98 views)

Mikrosatelity

Mikrosatelity

SINE. Mikrosatelity. A. G. SNPs : nuclear genome (consensus). S ingle n ucleotide p olymorphisms ( SNP s). Př.: chromozóm 1. Single-locus genetic markers. SNPs (single nucleotid e polymorphisms) – sekvenční polymorfismus

By kimi
(126 views)

PCR- Polymerase chain reaction

PCR- Polymerase chain reaction

PCR- Polymerase chain reaction PCR is an in vitro technique for the amplification of a region of DNA which lies between two regions of known sequence. PCR amplification is achieved by using oligonucleotide primers.

By coyne
(170 views)

Results Addition of PCR enhancing agents

Results Addition of PCR enhancing agents

4 SDS treatment [6]. 5 Microwave oven [7]. 6 FPFD c [8]. Add 30 µL 2 g SDS/L to tube. Add some cells to tube. Add 100 µL EB to tube d. Pick a colony and resuspend. Heat in microwave for 1 min e. Pick a colony and resuspend. Vortex vigorously. Add 25 µL PCR mix.

By lilac
(51 views)

Quantification of RNA by real-time PCR

Quantification of RNA by real-time PCR

Quantification of RNA by real-time PCR. Vilborg Matre. Overview. Real-time PCR. Gene expression profile - to characterize how a cell/animal responds to a stimulus. Replaces Northerns. Conventional PCR. One cycle. Denaturation. = a cyclic process leading to exponential

By kedem
(126 views)

Complex Experimental Design and Simple Data Analysis: A Pharmaceutical Example

Complex Experimental Design and Simple Data Analysis: A Pharmaceutical Example

Complex Experimental Design and Simple Data Analysis: A Pharmaceutical Example. Joseph G Pigeon Villanova University. Introduction. Designs with restricted randomization have multiple error measures Pharmaceutical example where the split plot structure is even more complex

By lilli
(74 views)

PCR & Sequencing

PCR & Sequencing

PCR & Sequencing. polymerase chain reaction ( PCR). produce many copies of a specific target segment of DNA. thermocycler. dNTPs (free nucleotides) Taq polymerase buffer 2 DNA primers. Figure 13.25. 2. 1. 3. Technique. 3 . 5 . PCR

By leda
(123 views)

Alberto Del Rosario Harlem Children Society, 3 rd year Memorial Sloan-Kettering Cancer Center

Alberto Del Rosario Harlem Children Society, 3 rd year Memorial Sloan-Kettering Cancer Center

Cloning of recombinant expression vectors that encode Interleukin-15 (IL-15), Interleukin-21 (IL-21) and Luciferase for tumor immunology studies. Alberto Del Rosario Harlem Children Society, 3 rd year Memorial Sloan-Kettering Cancer Center Manhattan Center for Science and Mathematics

By xanthe
(114 views)

View Pcr PowerPoint (PPT) presentations online in SlideServe. SlideServe has a very huge collection of Pcr PowerPoint presentations. You can view or download Pcr presentations for your school assignment or business presentation. Browse for the presentations on every topic that you want.