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- Unicellular protists - Poorly described taxonomic group

Transcriptomic characterization of Quahog Parasite Unknown (QPX) and the effects of temperature on gene expression. Annie Page-Karjian, Maya Groner, Jamie Sziklay, Ana Elisa Garcia, Ashton Griffin, Gregor-Fausto Siegmund, Sonia Singhal, Steven Roberts. Thraustochytrids.

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- Unicellular protists - Poorly described taxonomic group

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  1. Transcriptomic characterization of Quahog Parasite Unknown (QPX) and the effects of temperature ongene expression Annie Page-Karjian, Maya Groner, Jamie Sziklay, Ana Elisa Garcia, Ashton Griffin, Gregor-Fausto Siegmund, Sonia Singhal, Steven Roberts

  2. Thraustochytrids - Unicellular protists - Poorly described taxonomic group - Mostly saprophytes, but includes some highly virulent pathogens

  3. QPX Pathology - Massive inflammatory response - Swelling and nodules mantle and labial palps - Slower growth

  4. Perrigault M et al. 2010

  5. Environment Host Disease Pathogen

  6. Brothers et al. 2000

  7. Objectives of our study 1. Sequence and annotate the QPX transcriptome 2. Evaluate the effects of temperature on QPX gene expression 3. Identify biological function of enriched genes

  8. 10 ˚C 21 ˚C 45,082,610 reads 30,035,397 reads Illumina Sequencing De novo assembly 11,280 contigs RNA seq 2x Enrichment analysis 613 genes are differentially expressed and associated with enriched processes!

  9. Objectives of our study 1. Sequence and annotate the QPX transcriptome 2. Evaluate the effects of temperature on QPX gene expression 3. Identify biological function of enriched genes

  10. Results: Transcriptome characterization

  11. Results: Transcriptome characterization

  12. Objectives of our study 1. Sequence and annotate the QPX transcriptome 2. Evaluate the effects of temperature on QPX gene expression 3. Identify biological function of enriched genes

  13. Results: Differential gene expression

  14. Objectives of our study 1. Sequence and annotate the QPX transcriptome 2. Evaluate the effects of temperature on QPX gene expression 3. Identify biological function of enriched genes

  15. RESULTS: Enrichment analysis Fold increase Different biological processes

  16. RESULTS: Genetic connections between processes Developmental processes (4) Protein metabolism (67) Death (2) Cell adhesion (1) DNA metabolism (9) Transport (49) Stress response (13) Cell cycle and proliferation (14) RNA metabolism (110) Cell organization and biogenesis (10) Signal transduction (6)

  17. Protein Folding Ribosomal Biogenesis Lipid Biosynthesis RESULTS: THE PHYSIOLOGICAL LINK Brothers et al. 2000

  18. BONUS!

  19. BONUS!Genome characterization QPX Phytophthora

  20. Conclusions

  21. Environment Host Disease Pathogen

  22. Acknowledgements

  23. Questions?

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