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Biotechnology - PowerPoint PPT Presentation

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Biotechnology
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  1. Biotechnology

  2. DNA Identification • Only about 0.1% of DNA is different between individuals in the human population • This allows scientists to be able to identify people by their DNA---focus on the differences.

  3. Noncoding DNA • 98% of our DNA does not code for any proteins-Noncoding DNA • These regions contain variable number tandem repeats (VNTR). • CACACA,CACACA,CACACA,CACACA= ind. #1 • CACACA,CACACA,CACACA,CACACA,CACACA =ind. #2 • Geneticists calc. how frequently each of the repeats occur in the general population.-how rare it is.

  4. By identifying the VNTR’s at a number of locations in a person’s genome, a DNA profile can be developed for each individual in the population that is unique.

  5. P C R

  6. Restriction Enzymes • Nature’s Scissors

  7. Because each individual will have a unique number of places that restriction enzymes can make cuts, each person will have a unique set of DNA fragments of varying lengths. • How can scientists use this to make a DNA fingerprint?

  8. DNA Fingerprinting

  9. How can DNA finger-printing be used?