cancer cell chemotaxis in microfluidic devices l.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
Cancer Cell Chemotaxis in Microfluidic Devices PowerPoint Presentation
Download Presentation
Cancer Cell Chemotaxis in Microfluidic Devices

Loading in 2 Seconds...

play fullscreen
1 / 19

Cancer Cell Chemotaxis in Microfluidic Devices - PowerPoint PPT Presentation


  • 158 Views
  • Uploaded on

Cancer Cell Chemotaxis in Microfluidic Devices. Dallas Reilly, Chemistry Carthage College Faculty Mentor: Noo Li Jeon, Biomedical Engineering University of California, Irvine. Chemotaxis-movement of cells due to a chemical presence. Basics Cancer Breast Cancer Epidermal Growth Factor

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Cancer Cell Chemotaxis in Microfluidic Devices' - loring


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
cancer cell chemotaxis in microfluidic devices

Cancer Cell Chemotaxis in Microfluidic Devices

Dallas Reilly, Chemistry

Carthage College

Faculty Mentor: Noo Li Jeon, Biomedical Engineering

University of California, Irvine

Chemotaxis-movement of cells due to a chemical presence

outline
Basics

Cancer

Breast Cancer

Epidermal Growth Factor

Metastasis

Microfluidic devices can help

Microfluidic Devices

What are they?

How I use them

My Research

Outline

Experiments

Results

Conclusions

Acknowledgements

References

Outline
what is cancer
“Cancer” is a wide range of diseases involving irregular growth of cells

Normal cells vs. Cancer cells

What is Cancer?

Cancerhelp.org

epidermal growth factor egf
Epidermal Growth Factor (EGF)
  • What is it?
  • EGF and EGF receptor
  • What happens to a cell that binds to EGF?
  • Cancer and EGF

Wikipedia.org

metastasis

Vitalex

Metastasis
  • Metastasis- cancer cell migration
    • Tumors
    • Cancer cells move through the bloodstream and lymphatic system
  • EGF and metastasis
  • MDA-MB 231 Metastatic Breast Cancer Cells

Gary Carlson

microfluidics can help
Microfluidics can help
  • Microfluidic devices:
    • Gradients
    • Advantages
    • Modeling the body
  • If scientists can discover how and why cancer cells migrate we can start creating chemicals that stop or prevent them from doing so
what is a microfluidic device
What is a Microfluidic Device?
  • Many uses and applications: pharmaceuticals, biotechnology, the life sciences, defense, public health, and agriculture
  • Polydimethylsiloxane (PDMS) and soft lithography
  • Gradients

George Whitesides group, Harvard

Saadi, Jeon, Wang, Lin

slide9
Cont.
  • Procedure
    • 10X Hoffman
    • 3 Hours
    • Bell-curve gradient
  • Analysis
    • Metamorph
    • Excel
    • Oriana

Metamorph images

slide11

7/18/06, 7/21/06, 7/25/06

Summary

Conditions: 50ng/mL EGF, cells not starved

Average P-value: .0115

Average Degree (R/L): 97/262

CI Value (R/L): .13/.12

Standard Deviation (R/L): .12/.04

LHG

43/66 cells moved towards gradient-65%

48/64 cells moved towards gradient-75%

91/130-69%

EGF Gradient

slide12

7/28/06 and 8/1/06

Summary

Conditions: 500ng/mL EGF, cells not starved

Average P-value: . 612

Average Degree (R/L): 209/154

Average CI Value (R/L): .38/.01

Average Standard Deviation (R/L): .44/.24

LHG

18/44 cells moved towards gradient-41%

25/51 cells moved towards gradient-49%

43/95-45%

EGF Gradient

slide13

8/7/06

2 Exp.

Summary

Conditions: 50ng/mL EGF, cells starved (~12 hours)

Average P-value: . 695

Average Degree (R/L): 217/23

Average CI Value (R/L): .33/.07

Average Standard Deviation (R/L): .48/.18

RHG

8/19 cells moved towards gradient-42%

12/24 cells moved towards gradient-50%

20/43-47%

EGF Gradient

slide14

8/8/06

Exp. 3

Conditions: 50ng/mL EGF, cells starved (~5 hours)

8/11 cells moved towards gradient-72.7%

4/5 cells moved towards gradient-80%

12/16-75%

EGF Gradient

slide15

8/24/06 and 8/25/06

3 Exp.

Summary

Conditions: 50ng/mL EGF, cells starved (1-3 hours)

Average P-value: .016

Average Degree (R/L): 106/260

CI Value (R/L): .12/.09

Standard Deviation (R/L): .08/.06

RHG

31/46 cells moved towards gradient-67%

36/55 cells moved towards gradient-65%

67/101-66%

EGF Gradient

conclusions
Conclusions
  • EGF
  • High Concentration
  • Starvation
the future and present
The Future (and present)
  • More growth factors
  • Cells
  • Chemo-repellants
  • Devices that better model the body
  • Extracellular matrices
acknowledgements
Acknowledgements
  • I’d like to thank my mentor, Noo Li Jeon, my graduate student, Carlos Huang, and the rest of the great people in my lab for teaching me such an incredible amount in such a short time and for taking their time to assist my research
  • I’d also like to thank the UROP program, with which this research opportunity would have never been possible, their time and effort
references and works cited
References and works cited:

George M. Whitesides. The Origins and the Future of Microfluidics. Nature Publishing Group, July 2006.

George M.Whitesides, Emanuele Ostuni, Shuichi Takayama, Xingyu Jiang, and Donald E. Ingber. Soft Lithography in Biology and Biochemistry. Annual Review of Biomedical Engineering, 2001 (335-573).

Noo Li Jeon, Harihara Baskaran, Stephan K.W. Dertinger, George M. Whitesides, Livingston Van De Water, and Mehmet Toner. Neutrophil Chemotaxis in Linear and Complex Gradients of Interleukin-8 Formed in a Microfabricated Device. Nature Publishing Group, 2002.

Stephan K. W. Dertinger, Daniel T. Chiu, Noo Li Jeon, and George M. Whitesides. Generation of Gradients Having Complex Shapes Using Microfluidic Networks. Analytical Chemistry (ACS), 2001.

Noo Li Jeon, Stephan K. W. Dertinger, Daniel T. Chiu, Insung S. Choi,

Abraham D. Stroock, and George M. Whitesides. Generation of Solution and Surface Gradients Using Microfluidic Systems. Langmuir (ACS), 2000.

Shur-Jen Wang, Wajeeh Saadi, Francis Lin, Connie Minh-Canh Nguyen, Noo Li Jeon. Differential Effects of EGF Gradient Profiles oN MDA-MB-231 Breast Cancer Cell Chemotaxis. Elsevier, INC, 2004.

Wajeeh Saadi · Shur-Jen Wang · Francis Lin · Noo Li Jeon. A Parallel-Gradient Microfluidic Chamber for Quantitative Analysis of Breast Cancer Cell Chemotaxis. Biomedical Devices (109-118), 2006.

Jennifer Ouellette. A new wave of microfluidic devices. The Industrial Physicist (14-17), 2003.

Laurie Tarkan. Scientists Begin to Grasp the Stealthy Spread of Cancer. The New York Times, 2006.