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Exploring Cell-Cell Communication and Sensing in Cuttlefish and Vibrio Harveyi

This study delves into the fascinating realm of cell-cell communication and chemotaxis using Cuttlefish and Vibrio Harveyi as natural models. By investigating signaling cascades and chimeric receptor systems, the research sheds light on the intricate mechanisms governing interactions between prey and predator cells. Through enzyme-mediated autoinducer production and receptor-triggered chemotaxis, a deeper understanding of how organisms communicate and respond to stimuli is uncovered. This exploration offers insights not only into marine ecosystems but also into the broader implications for synthetic biology and bioengineering projects.

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Exploring Cell-Cell Communication and Sensing in Cuttlefish and Vibrio Harveyi

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  1. Cell-Cell Communication, Sensing and Chemotaxis N Kuldell for 20.020 Spring 2009

  2. Heidelburg iGEM 2008: ecolicense to kill Part 1: Sensing Background information

  3. Cell-Cell Communication natural context: Cuttlefish and Vibrio harveyi Cuttlefish: master of camouflage During the day they lie buried in the bottom of the ocean; at night they swim and hunt for food.

  4. AI-2 AI-1 Cell-Cell Communication natural context: Cuttlefish and Vibrio harveyi Few cells: Autoinducers diffuse away More cells: Autoinducers initiate signaling, transcription Photo from B. Bassler, Fig from: Genes & Dev. 2001. 15: 1468-1480 doi: 10.1101/gad.899601

  5. Cell-Cell Communication iGEM context: “prey” pathogen, “predator” killer Prey Cell LuxS= enzyme that produces autoinducer 2 (AI2)…diffuses through the media Predator Cell Nature (2002) 415: 545 LuxP, LuxQ = receptor that binds (AI2)…induces chemotaxis….how?

  6. AI-2 AI-1 Sensing and Signaling Cascades natural context: “TCS” 2 component signaling Stimulus Activates SHK Transfers to RR Output P TCS “SHK” sensor histidine kinase “RR” response regulator

  7. Sensing and Signaling Cascades natural context: “TCS” 2 component signaling Stimulus Activates SHK Transfers to RR Output P TCS “SHK” sensor kinase “RR” response regulator Plant Physiol. (1998) 117: 723

  8. Sensing and Signaling Cascades natural context: “TCS” 2 component signaling Stimulus Activates SHK Transfers to RR Output Tar Che flagella P CCW, default, tumble CW, signal, swim TCS “SHK” sensor kinase “RR” response regulator J Bacteriol. (2000)182: 6865

  9. Sensing and Signaling Cascades iGEM context: chimeric receptor

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