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Safe Working Practices for Honours, Directed Studies Students. General Safety Precautions. General Rules Lab Coat Footwear Handwashing No Eating in Lab Area Personal Belongings Fire Procedure Injuries Emergency Number. Biohazardous Materials. Do not remove plates etc from lab
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Safe Working Practices for Honours, Directed Studies Students
General Safety Precautions • General Rules • Lab Coat • Footwear • Handwashing • No Eating in Lab Area • Personal Belongings • Fire Procedure • Injuries • Emergency Number
Biohazardous Materials • Do not remove plates etc from lab • Waste plates • Waste non disposals • Spills • Nothing Goes Down The SINK Unprocessed • Disinfect work area • Spill cleanup • Wash hands before leaving
Chemical Preparation • Check MSDS for proper handling • Labeling and storage • PPE • Order needed • Safe Disposal
Disposal • Glassware • Broken glass • Autoclaving • Hazardous wastes
Glassware • Rinse and ensure glassware is immersed in water in bucket in washup area • When necessary do a load in dishwasher • Return to appropriate shelf etc
Broken Glass • Not contaminated with biohazard in glass breakage containers • Biohazard contaminated: yellow sharps containers • Leave note on white board in S365C if broken glassware
Autoclaving • Waste biohazards • Liquids • Use slow exhaust • Wrapped • Allow for drying cycle • Autoclave tape • Check with others before starting (particularly Carolynne)
Hazardous Waste • Container • Appropriate size • Labeling • Contains all ingredients (no abbreviations) • Location • S365F
Before Leaving After Every Session • Clean any areas that have been used (balances, counter, fumehood) • Glassware is put in washup area • Turn off gas • Turn off deionized water • Turn off lights • Turn off autoclave • Lock door
Working Alone • If at all possible avoid it • A risk assessment of your work must be done with your supervisor to determine what can be done with minimum supervision • Higher risk procedures should not be done when alone (working with hazardous materials, risk of fire) • http://www.ccohs.ca/oshanswers/hsprograms/workingalone.html
Working Alone • The Faculty of Science will be striking a multidiscipline committee to develop working alone guidelines for the Faculty of Science • Have some type of checkin procedure
Safe Work Procedures • http://www.tru.ca/hsafety/workinglearningsafely/work.html
What is WHMIS • Workplace Hazardous Materials Information System • Provides information about hazardous materials used in the workplace • Workers have the right to receive information about each controlled product they use • Goal of WHMIS is to reduce injury and disease.
HAZARD CLASSES • Class A: Compressed Gas • Class B: Flammable & Combustible • Class C: Oxidizing Material • Class D: Poisonous & Infectious Material
HAZARD CLASSES • Class E: Corrosive Material • Class F: Dangerously Reactive Material
SUPPLIER LABELS Seven items of information must be included on supplier label: • Product identifier • Hazard Symbols • Risk Phrase • Precautionary Statements • First Aid Measures • Supplier Identification • Reference to MSDS
WORKPLACE LABELS Required on containers for each controlled product on site, including secondary containers. Provide 3 types of information: • Product Identifier • Safe Handling and personal protective clothing and equipment. • Reference to MSDS
MSDS SHEET • Material Safety Data Sheet • provides specific hazard information, safe handling information, and emergency procedures for a controlled product. • Key source of information for developing safe working procedures.
MSDS SHEETS Nine Recommended Section Headings: • Product Information • Hazardous Ingredients • Physical Data • Fire and Explosion Hazard • Reactivity Data • Toxicological Properties • Preventative Measures • First Aid Measures • Preparation Information
Cycloheximide MSDS • Routes of Entry: Absorbed through skin. Eye contact. Inhalation. Ingestion. • Toxicity to Animals: Acute oral toxicity (LD50): 2 mg/kg [Rat]. • Chronic Effects on Humans: • DEVELOPMENTAL TOXICITY: Classified Reproductive system/toxin/female, Development toxin [POSSIBLE]. • Causes damage to the following organs: the nervous system, liver.
Cycloheximide • May cause damage to the following organs: blood, kidneys, gastrointestinal tract, skin. • Other Toxic Effects on Humans: Very hazardous in case of skin contact (irritant), of ingestion, of inhalation.
TRU MSDS • Bookmark student computer S365B • ThompsonMSDS • Msds • Read before using new chemicals
RESPONSIBLITIES Workers • Know & understand info on labels and MSDS • Handle controlled products safely. • Inform employer if labels missing Employer • Ensure workers understand WHMIS • Provide training • Ensure controlled products have MSDS and WHMIS Labels • MSDS available to workers