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Supplemental Figure 1 - PowerPoint PPT Presentation


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Supplemental Figure 1. ES. EB Stage. Tertiary Culture Stage. Colony Formation. Serum. Serum Replacement. Semi-solid culture. D7-3 o. D10-3 o. D2 EB. D6 EB. Add NEA. Fluorescence based Sorting. High MTG. Low MTG.

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slide1

Supplemental Figure 1

ES

EB Stage

Tertiary Culture Stage

Colony Formation

Serum

Serum Replacement

Semi-solid culture

D7-3o

D10-3o

D2 EB

D6 EB

Add NEA

Fluorescence based

Sorting

High MTG

Low MTG

Supplemental Figure 1. Protocol for differentiation of ES cells to insulin-expressing colonies. ES: embryonic stem cells; EB: embryoid body; MTG: monothioglycerol; NEA: a combination of nicotinamide, exendin-4 and activin B.