Supplemental Figure 1. ES. EB Stage. Tertiary Culture Stage. Colony Formation. Serum. Serum Replacement. Semi-solid culture. D7-3 o. D10-3 o. D2 EB. D6 EB. Add NEA. Fluorescence based Sorting. High MTG. Low MTG.
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Tertiary Culture Stage
Supplemental Figure 1. Protocol for differentiation of ES cells to insulin-expressing colonies. ES: embryonic stem cells; EB: embryoid body; MTG: monothioglycerol; NEA: a combination of nicotinamide, exendin-4 and activin B.