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Cellular Respiration

This laboratory experiment investigates cellular respiration rates in germinated and non-germinated peas. Using CO2 sensors and a respiration chamber, we will measure carbon dioxide concentrations to analyze respiration under various temperature conditions. The experiment will compare the respiration rates, exploring how germination affects cellular processes. Students will gain hands-on experience with Logger Pro software, analyze O2 levels, and observe pressure changes during respiration, contributing to a deeper understanding of the fundamental biological process of cellular respiration.

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Cellular Respiration

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  1. Cellular Respiration Sita M Damaraju (Fellow)

  2. Glucose + Oxygen → Carbon dioxide + Water + Energy (ATP) 6H12O6 + 6O2 → 6CO2 + 6H2O + ATP

  3. Vernier Lab Part II • Determine whether germinated or non-germinated peas respire. • Use a CO2 gas sensor to measure concentrations of carbon dioxide. • Study the effect of temperature on cell respiration. • Compare the rates of cell respirations in germinated peas and non-germinated peas.

  4. Materials • CO2 sensor • 250mL respiration chamber Computer • Logger pro software • Lab pro (sensor-computer interface) • 25 germinating peas • 25 non-germinating peas • Thermometer

  5. Set up CO2 sensor Germinated Peas Lab pro Non germinated peas

  6. Results

  7. Extensions • Measure O2 concentration using germinated and non germinating peas at different temperatures • Measure pressure changes during respiration of germinating and non-germinating peas at different temperatures (AP Biology lab) • Simultaneously measure O2 and CO2 concentrations at different temperatures for germinating and non-germinating peas.

  8. Sensors • CO2 sensor • O2 sensor • Gas pressure sensors

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