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Centrifuge – DS-101

Centrifuge – DS-101. CHEE 450 March 27 2009. Team 7 Norman Li, Joe Steele, Ivy Wang. From Fermentation. Removal of 93% of incoming water. Removal of 77% of incoming water. OVERALL. Biomass Glucose Salts Water IBs WFI Mass/batch. 2.95% 4% 0.58% 92.16% 0% 0% 38010 kg.

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Centrifuge – DS-101

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  1. Centrifuge – DS-101 CHEE 450 March 27 2009 • Team 7 • Norman Li, Joe Steele, Ivy Wang

  2. From Fermentation Removal of 93% of incoming water Removal of 77% of incoming water OVERALL Biomass Glucose Salts Water IBs WFI Mass/batch 2.95% 4% 0.58% 92.16% 0% 0% 38010 kg 0.03% 0.26% 0.04% 11.7% 14.64% 71.84% 1442 kg To Reactions Inclusion Bodies (IB) originally contained in biomass Removal of 70% of incoming water

  3. What “Force”d the Separation? • Centrifugal Force is not really a force • The centrifuge operates based on Centripetal Force

  4. Main Features of Disc Stack Centrifuge & Their Roles • Inlet • Distributor • Disc stack • Pairing disc • Solid ports • Vertical spindle

  5. Centrifugation Alternatives • Two common cell separation techniques • Filtration or Centrifugation

  6. Designing a Centrifuge • Based on empirical data and previous experience • Supply process data to supplier Utilities Consumption Separation Efficiency Throughput

  7. Considerations forBiological Systems • Sterilization • High pressure steam • FDA approved EPDM gaskets • Sterile Operation • Shear Forces

  8. BTPX 710 Separation System • 0.5-500 µm • Partial discharge • High-grade SS • Whole-system 3 bar steam sterilization • Fully contained • Complete system • Control • Pumps • Valves

  9. BTPX 710 Separation System

  10. BTPX 710 Separation System • Martin Ivanov, P.Eng., Alfa Laval Canada • $1.6M CAD • Additional Costs • $150-250,000 for certification and testing • FAT – Factory Acceptance Testing • cGMP – Current Good Manufacturing Practices

  11. Questions? Science and Mechanics Magazine – Aug. 1935

  12. References Alfa Laval (n.d) BTPX separator – How it works. Mar. 21, 2009 from Alfa Laval website. http://www.alfalaval.com/solution-finder/products/btpx-separator/pages/howitworks.aspx Alfa Laval – disc stack centrifuge technology. Controlling the separation of liquid and solids [Brochure]. Ambler CM (1959) The theory of scaling up laboratory data for the sedimentation type centrifuge. J. Biochem. Microbiol. Technol. Eng. 1:185-205. Axelsson H (1999) Cell Separation: Centrifugation. In: Flickinger MC, Drew SW (eds). Encyclopedia of Bioprocess Technology. John Wiley & Sons, New York. pp 513-530. Couper JR, Penney WR, Fair JR, Walas SM (2005) Chemical Process Equipment: Selection and Design, 2nd Ed. Elsevier Inc,Oxford. pp 357. GEA Filtration (n.d) Benefits in using Microfiltration for enzyme cell separation. Mar. 20, 2009 from GEA Filtration website. http://www.geafiltration.com/library/enzyme_cell_separation.asp New Brunswick (n.d) CEPA High Speed Centrifuges. Mar. 20, 2009. from New Brunswick website. http://www.nbsc.com/cepa.aspx

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