Ch. 5 Molecular Tools

Jul 23, 2012

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Ch. 5 Molecular Tools

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Molecular Tools and Technologies

Molecular Tools and Technologies Nathaniel E. Ostrom Dept. of Zoology and Center for Global Change and Earth Observations, Michigan State University Tim Filley Dept. of Earth and Atmospheric Sciences, Purdue University Stefan Scherer

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molecular biology tools

General. mRNA copies of both prokaryotic and eukaryotic genes have long open reading frames (ORFs)Deciphering the information content in genomes is insurmountableBioinformaticians Small number of molecular biology toolsSix important molecular biology techniques. Restriction Enzyme Digestion. Isolated from bacterial cellsFunction in bacteria to defend again virusesMolecular scissors to cut DNARecognize palindromic sequences (same forward and reverse)Recognize 4 to 10 base pairs and cut p9460

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Ch # 5

Ch # 5. Atoms, Molecule, Formulas, Subatomic Particles. Atoms. An atom is the smallest particle of an element that can exist and still have properties of the element. Atomic Theory of Matter. All matter is made up of small particles called atoms.113 types.

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Ch 5 Lecture 1 Molecular Orbitals for Diatomics

Ch 5 Lecture 1 Molecular Orbitals for Diatomics. Introduction Molecular Orbital (MO) Theory uses Group Theory to describe bonding Simple Bonding Theories don’t explain all molecular properties MO Theory often better explains some properties (ex: magnetism) How MO Theory Works

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CH 5

CH 5. Enculturation. Enculturation:. The process by which children learn and adopt the ways, manners and values of their culture. Culture:

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Ch 5

Ch 5. How Ecosystems work. Energy in Ecosystems. What is the source of energy for most ecosystems? The sun! What kingdom of organisms use this energy first ON LAND? Plants What kingdom of organisms use this energy first IN WATER? Protists: Algae

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Ch. 5

Ch. 5. Assessment- page 300/301. Q. 1. Military leader of Japan?. A. 1. Shogun. Q. 2. Artisans/merchants formed ? for protection/profit?. A. 2. guilds. Q. 3. Yayoi formed ? which headed by small group of warriors?. A. 3. Clans. Q. 4. In ?, person clears mind of all thoughts/desires.

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Ch. 5

Subject Verb Agreement . Ch. 5. Agreement of Subject and Verb. A verb should agree in number with its subject. Singular subjects take singular verbs, and plural subjects take plural verbs. In a verb phrase, the first helping verb agrees in number with the subject

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Ch 21: Molecular Evolution

Ch 21: Molecular Evolution. Genomes and Their Evolution. An organism’s genome is the full set of genes it contains. In eukaryotes, most of the genes are found in the nucleus, but genes are also present in plastids, mitochondria and chloroplasts.

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CH 10: Molecular Geometry

CH 10: Molecular Geometry. Renee Y. Becker Valencia Community College CHM 1045. Molecular Shapes: VSEPR. The approximate shape of molecules is given by Valence-Shell Electron-Pair Repulsion (VSEPR) . Step 01: Count the total electron groups.

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Ch. 5

Ch. 5. Earthquakes. I. Over millions of years, 3 types of stress change the shape & volume of rock. A. Tension – pulls or stretches crust 1. occurs where plates are moving apart. B. Compression – squeezes crust together 1. plates push against each other until

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CH. 5

CH. 5. R I G H T S. H U M A N. HUMAN RIGHTS KEY TERMS. HUMAN RIGHTS Fundamental rights to which all people are entitled. STEREOTYPING Having a simplified fixed judgment on a group of people. DISCRIMINATION Treating a person unfairly because of race, religion, ability, etc.

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Ch 5

Ch 5. Biosocial development: First two years. Early Physical Development. Principles of Development. Cephalocaudal principle: principle that development proceeds in a head-to-tail direction, upper parts of body develop before lower parts

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Molecular Tools: Molecular Separations *Gel Electrophoresis

Molecular Tools: Molecular Separations *Gel Electrophoresis. Molecular Tools: Molecular Separations *Gel Electrophoresis *One- and Two-Dimensional Gel Electrophoresis of Proteins. Molecular Tools: Molecular Separations *Gel Filtration Chromatography. Molecular Tools:

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CH 5

CH 5. Project Scope and Human Resources Planning. Objectives. Acquire a general understanding of the parts of the project management plan Understand the importance of discovering and documenting stakeholder requirements Understand how to create a detailed scope statement and WBS

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Ch 12 Molecular Geometry

Ch 12 Molecular Geometry. Lewis Dot Structures do not represent the actual shape of molecules. However, Lewis Dot Structures do allow us to predict the shape of the molecule. Predicting the 3-dimensional shape of a molecule is called molecular geometry.

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Ch 6.5 Molecular Geometry

Ch 6.5 Molecular Geometry. VSEPR Theory. Use VSEPR theory Valence-Shell-Electron-Pair Repulsion =the repulsion between electron pairs causes molecular shapes to adjust so that the valence-electron pairs stay as far apart as possible.

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CH 5

CH 5. Integration 積分. 學習內容. 面積與距離. Area and Distance The Definite Integral The Fundamental Theorem of Calculus Indefinite Integrals and the Net Change Theorem Tne Substitution Rule. 定積分. 微積分基本定理. 不定積分. 代換法. 5.2. The Definite Integral 定積分. 學習重點. 用定積分符號表示不規則區域的面積知道均值定理

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Ch 5

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Molecular Tools: Engineering Applications

Molecular Tools: Engineering Applications. Environmental Engineering: State of the Art. “Sanitary Engineering” “Environmental Engineering” Biological Processes: Wastewater BOD removal Nutrient Removal Bioremediation Primary Substrate Degradation: ex: oil spills

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Ch. 8 Molecular Shapes

Ch. 8 Molecular Shapes. aka MOLECULAR GEOMETRY. MOLECULAR GEOMETRY. Molecules adopt the shape that minimizes the electron pair repulsions. VSEPR V alence S hell E lectron P air R epulsion theory .

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Molecular Tools

Molecular Tools. Recombinant DNA. Restriction enzymes Vectors Ligase and other enzymes. DNA Cloning. Restriction enzymes Plasmid vector Selectable gene Cloning sites Origin of replication DNA ligase Transformation Competent cells Selection. Dideoxy DNA Sequencing.

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