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Advanced Molecular Tools for DNA Cloning and Sequencing

Explore the power of recombinant DNA techniques featuring restriction enzymes, vectors, ligase, and other enzymes for DNA cloning. Learn about dideoxy DNA sequencing with automated sequencers and Polymerase Chain Reaction with thermostable DNA polymerase. Discover the secrets of plasmid vectors, selectable genes, and competent cells in genetic engineering. Transform your understanding of molecular biology with this comprehensive overview of essential tools and techniques.

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Advanced Molecular Tools for DNA Cloning and Sequencing

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  1. Molecular Tools

  2. Recombinant DNA • Restriction enzymes • Vectors • Ligase and other enzymes

  3. DNA Cloning • Restriction enzymes • Plasmid vector • Selectable gene • Cloning sites • Origin of replication • DNA ligase • Transformation • Competent cells • Selection

  4. Dideoxy DNA Sequencing • Dideoxy nucleotides • Automated DNA sequencers

  5. Polymerase Chain Reaction

  6. Polymerase Chain Reaction • Thermostable DNA polymerase • Oligonucleotide primers • NTPs + buffer • PCR machine

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