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Molecular Tools: Molecular Separations *Gel Electrophoresis

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  1. Molecular Tools: Molecular Separations *Gel Electrophoresis

  2. Molecular Tools: Molecular Separations *Gel Electrophoresis *One- and Two-Dimensional Gel Electrophoresis of Proteins

  3. Molecular Tools: Molecular Separations *Gel Filtration Chromatography

  4. Molecular Tools: Labeled Tracers *Autoradiography *Phosphorimaging

  5. Molecular Tools: Nonradioactive Tracers

  6. Molecular Tools: Nucleic Acid Hybridization * Southern Blots H E B H E B H E B H E B kb 23.1 9.45 6.56 4.35 2.32 2.02 0.56 P3 P2b P1 P2a

  7. Molecular Tools: Nucleic Acid Hybridization *DNA Fingerprinting/Typing

  8. Molecular Tools: Nucleic Acid Hybridization *In Situ Hybridization Minisatellite Telomeres Glycogen phosphorylase gene Sykorova, et al. (2006) Am. J. Bot. 93(6) 814-823

  9. Molecular Tools: Immunoblots (Western Blots) Isolate Ribosomes SDS-PAGE S6 Y Transfer to nitrocellulose Incubate with Antisera P3 Y

  10. Molecular Tools: Sanger dideoxy DNA sequencing

  11. Molecular Tools: Pyrosequencing (454 sequencing)

  12. Molecular Tools: Site-Directed Mutagenesis

  13. Endosperm 15 20 25 Aleurone 15 20 25 15 20 25 Embryo Coleoptile Root Leaf Ear Pollen Silk DPP P1 P2a P2b 18 S rRNA P3 Molecular Tools: Northern Blots

  14. Fig. 5.34 Molecular Tools: Quantitative PCR concepts

  15. Molecular Tools: Quantitative PCR quantification

  16. Molecular Tools: Reporter Gene Transcription