spectrophotometry chapter 17 harris
Skip this Video
Download Presentation
Spectrophotometry Chapter 17, Harris

Loading in 2 Seconds...

play fullscreen
1 / 25

Spectrophotometry Chapter 17, Harris - PowerPoint PPT Presentation

  • Uploaded on

Spectrophotometry Chapter 17, Harris. Spectrophotometry is the use of the measurement of the interaction of Electromagnetic radiation (EMR) with matter to quantize the concentration of an analyte. There are many different types of

I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
Download Presentation

PowerPoint Slideshow about 'Spectrophotometry Chapter 17, Harris' - cortez

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.

- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
spectrophotometry chapter 17 harris
SpectrophotometryChapter 17, Harris

Spectrophotometry is the use of the measurement of the interaction of Electromagnetic radiation (EMR) with matter to quantize the concentration of an analyte. There are many different types of

spectrophotometers, based on the wavelength region of the EMR they measure. Examples are uv-vis, IR, microwave, x-ray, etc.


electromagnetic radiation
Electromagnetic Radiation

Electromagnetic Radiation travels at the speed of light (c), 2.997 x 108 m/s

Monochromatic light has a very small wavelength spread or narrow bandwidth; one 

Polychromatic light has several wavelengths or  in its beam.

electromagnetic radiation1
Electromagnetic Radiation

Frequency (, Greek nu): Number of peaks that pass a given point per unit time.

Wavelength (, Greek lambda): Distance from one wave peak to the next.



 = 1/, cm-1

Amplitude: Height measured from the center of the wave. The square of the amplitude gives intensity.

Plane polarized EMR consists of the sinusoidal electric field vectors in one plane with magnetic field vectors orthogonal to the electric field vectors. The above wave is traveling in the x direction in the above diagram.
Frequency and wavelength are related by

c =  = 2.997 X 108m/s

Energy and frequency are related by the expression

E = h 

where h (Planck’s constant = 6.626 X 10-34 J s)

Depending on the specific region of the EMR, various units are used to express the 

 symbolRegion of EMR

meter m radio

millimeter mm microwave

micrometer m infrared

nanometer nm visible/ultraviolet

Angstrom (10-10 m) Å X-ray

picometer pm -ray

Different regions of the EM spectrum produce different types of transitions in molecules. Note the inverse relationship between wavelength and energy, i.e., the shorter the  the greater the Energy.
The absorption of EM radiation increases the energy of the molecule in one of the ways described on the proceeding slide. Emission results when the molecule loses energy.
The visible spectrum is a very narrow region of the EMR spectrum. Note that the shortest  is at the violet end of the visible spectrum. The ultraviolet region is to its left. The longest  at the red end of the visible spectrum, and the infrared region is to its right.
Fundamental to all of the spectroscopic methods is the quantization of energy.
  • Let’s consider an atom first.
  • Atoms exist in discrete (or quantized) potential energy (PE)
  • levels.
  • The PE depends on the electronic configuration of the atom
  • Transitions of outer shell electrons between definite levels
  • occur at definite “sized” energy according to E = h 
    • if E absorbed, the electron is excited to a higher level.
    • if E emitted, the electron falls to a lower level.
  • Thus, each transition occurs with a specific energy, or since
  • E = h = hc/, each transition gives rise to a specific spectral
  • line, either absorption or emission depending on the process.
Fundamental to all of the spectroscopic methods is the quantization of energy.

Now, for a molecule,

Energy TOTAL =Eelectronic + Evibrational + Erotational

Where Eelectronic occursin the UV-Vis

Evibrational in the IR, and

Erotational in the microwave region of the EMR

Electronic transitions are accompanied by fine structure, i.e., vibrational and rotational transitions.

The fine structure of vibrational transitions (v0 – v4 ) on the electronic E 0 – E1. Not shown would be the fine structure of rotational transitions within each vibrational level.
transmittance and absorbance
Transmittance and Absorbance

There are two quantities that relate the change in

the intensity of EM before and after interaction

with matter.

  • Transmittance = P / P0, and
  • Absorbance, defined as

A = log10 (P0 / P ) = - log10 (P/ P0 )

beer s law
Beer’s Law

Beer’s Law (or the Beer-Lambert Law) expresses the relationship between absorbance (A), the length of cell(b), and the concentration of the absorbing species (c). The proportionality constant is  and is known the molar absorptivity. This relationship is given by the equation

A =  b c

The absorption spectrum of a sunscreen lotion showing A as a function of  of the EM radiation. This spectrum is in the ultraviolet (UV) region. UV-B is the shorter  or higher energy and thus more harmful to skin.
Various typical cells (cuvets) used in spectrophotometry. The faces (part in the light path) may be silica, quartz, glass, or plastic, but must be transparent to the radiation of interest.
Analysis of analytes by spectrophotometry involves converting the analyte to a light-absorbing species. Pictured here is the chemistry for the analysis of the nitrite ion, NO2-.
Calibration curve for the analysis of nitrite. The  of 543 nm and b (the pathlength of the cuvet) are kept constant