Supplemental Figure 1

1. Supplemental Figure 1 a b d c * e f g Renal injury was evaluated in 12 male hemizygous transgenic mice of the C57Bl/6 strain and 13 age-matched wildtype littermates. (a) Renal PRR expression was 30-fold increased in the transgenic mice. However, no significant difference was found for (b) albuminuria and (c) glomerular injury as well as renal expression of (d) PAI-1, (e) CCL2, (f) TGF-beta and (g) collagen IV.Basal data of the mice are shown in the supplemental table below.*=p<0.0001 Basal data male Atp6ap2/PRR C57Bl/6 mice 26

2. Supplemental Figure 2 a DOCA + Ang II Normotensive Control C57Bl/6 b 5/6 Nephrectomy Control FVB/N Supplemental figure 2a shows that glomerular injury can be induced in C57Bl/6 mice. Administration of the aldosterone analogue DOCA in combination with infusion of Ang II by osmotic minipumps for two weeks induces arterial hypertension and severe glomerular injury as shown previously by us (Krebs). Moreover, as shown in supplemental figure 2b mice of the FVB/N strain are sensitive to 5/6 nephrectomy and develop severe glomerular injury within a few weeks after removal of one kidney and 2/3 of the remaining kidney as shown previously by us (Fraune). References: Krebs C, Fraune C, Schmidt-Haupt R, Turner JE, Panzer U, Nguyen Quang M, Tannapfel A, Velden J, Stahl RAK, Wenzel UO: CCR5 deficiency does not reduce hypertensive endorgan damage in mice. Am J Hypertension 25:479-86, 2012 Fraune C, Lange S, Krebs C, Hölzel A, Baucke J, Divac N, Schwedhelm E, Streichert T, Velden J, Garrelds IM, Danser AH, Frenay AR, van Goor H, Jankowski V, Stahl R, Nguyen G, Wenzel UO: AT1 antagonism and renin inhibition in mice: pivotal role of targeting angiotensin II in chronic kidney disease. Am J Physiol Renal Physiol 303:F1037-48, 2012