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Gel Electrophoresis Experiment Spring 2004

Gel Electrophoresis Experiment Spring 2004. A Timed Presentation - do not click the mouse. Approximate Run Time - 2 minutes 15 seconds. Comb produces 28 wells. Gel Apparatus. Restriction Enzyme Digest Oven. Adjusting the Micropipette. Loading DNA. Loading DNA. Loading DNA.

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Gel Electrophoresis Experiment Spring 2004

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  1. Gel Electrophoresis Experiment Spring 2004 A Timed Presentation - do not click the mouse. Approximate Run Time - 2 minutes 15 seconds

  2. Comb produces 28 wells Gel Apparatus

  3. Restriction Enzyme Digest Oven

  4. Adjusting the Micropipette

  5. Loading DNA

  6. Loading DNA

  7. Loading DNA

  8. Loading DNA

  9. Loading DNA

  10. 15 minutes 80 Volts

  11. 15 minutes 80 Volts View from above

  12. 45 minutes 80 Volts Note separation of die markers

  13. 2 hours 40 minutes 80 Volts Note separation of die markers

  14. 2 hours 40 minutes 80 Volts 3 hours 10 minutes 30 Volts 15 hours 35 minutes 25 Volts Note separation of die markers

  15. DNA Ladder used in all gel electrophoresis experiments

  16. Ladder Rows: 1 11 21 28

  17. Double Digest Rows: 2 To 10 EcoR1 & HindIII

  18. Single Digest Rows: 11 To 20 HindIII

  19. No Restriction Enzymes Used Rows: 22 To 27

  20. 850 bp

  21. 1650 bp

  22. 5000 bp

  23. 6000 bp

  24. Double Digest 900 bp sample 5100 bp sample Single Digest 6000 bp sample Uncut Plasmid Several smears

  25. The SBI4U class of Hill Park Secondary School wish to thank Dr. D. Tang (McMaster University/St. Joseph’s Hospital) and his lab technician for donating the DNA, Restriction Enzymes and Ladder and for calculating the amounts of material required to complete this procedure successfully.

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