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Vibrio Cholera. Michelle Ross, Kristin Roman, Risa Siegel. Vibrio Cholera. Micro and Molecular biology. Vibrio Cholera. Gram-negative Curved rod .5-.8 μ m width 1.4-2.6 μ m length Facultative anaerobe Single polar flagellum Chemoorganotroph Optimal growth 20-30 degrees. V. Cholera.

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vibrio cholera
Vibrio Cholera

Michelle Ross, Kristin Roman, Risa Siegel

vibrio cholera2

Vibrio Cholera

Micro and Molecular biology

vibrio cholera3
Vibrio Cholera
  • Gram-negative
  • Curved rod
  • .5-.8 μm width
  • 1.4-2.6 μm length
  • Facultative anaerobe
  • Single polar flagellum
  • Chemoorganotroph
  • Optimal growth 20-30 degrees
v cholera
V. Cholera

Gram-negative

  • lipopolysaccharide coat which provides protection against hydrophobic compounds
  • provides a surface for immune recognition
divisions of v cholera
Divisions of V. Cholera
  • Biotype (biovar)

different strains of the same bacterial species

distinguished by a group of phenotypic or genetic traits

  • Serogroup

bacteria of the same species with different antigenic

determinants on the cell surface

V. Cholera has more than 150 different serogroups, only two of which cause epidemic disease

v cholera 01 serogroup
V. Cholera01 serogroup

Classic

genome: 3.2-3.6 Mb

El Tor (El)

genome: 4 Mb

01 antigen is divided into 3 types: A,B,C

  • A antigen

made of 3-deoxy-L-glycerotetronic acid

  • B, C antigen

not been characterized

horizontal gene transfer
Horizontal Gene Transfer

1. acquisition of VPI

2. lysogenic conversion by phage

3. exchange of genes leads to expression of O-antigen and capsule

v cholera8
the 01 strain and the recent 0139 strain have different antigens expressed in the polysaccharide capsule

the change in structure is thought to have arisen from a recombination event.

V. Cholera
v cholera9
V. Cholera

two circular chromosomes

Chromosome 1 is larger (2.96

million base pairs) and carries

many genes for essential cell

functions and housekeeping

Chromosome 2 is smaller (about

1.07 million base pairs and

carries the integron island

chromosome 1
Chromosome 1
  • carries many genes for essential cell functions and housekeeping. It also contains important virulence genes, most of which have been acquired by lateral gene transfer from other species
  • chromosome one carries two bacteriophages.

ONE VIRUS is called the V. cholera pathogenicity island phage

(VPI), which infects and inserts its DNA into the bacterial

chromosome and allows the synthesis of a pilus which

the bacteria uses to attach to the host intestine

SECOND VIRUS is called the cholera-toxin phage (CTX).

The CTX phage inserts itself into chromosome

one and the bacterium is then capable of secreting

a powerful enterotoxin

chromosome 2
Chromosome 2

The integron region is often found on plasmids and serves as a "gene capture system." This region may contain antibiotic resistance genes.

pathogenesis of v cholera
Pathogenesis of V. Cholera

Cholera disease begins with ingestion of contaminated water or food. The bacteria that survive the acidic conditions of the stomach colonize in the small intestine.

The cholera toxin (CT) is responsible for the severe diarrhea characteristic of the disease.

Cholera Toxin

CT is a proteinaceous enterotoxin secreted by

V. Cholera

cholera toxin
Cholera Toxin

Structure

  • Composed of a AB subunit. The B subunit forms a pentameric “doughnut” like structure that binds the CT to the receptor on the eukaryotic cells

Pathway

  • The A subunit contains the enzymatically active portion or the toxin
  • Proteolytic cleavage of the A subunit results in A1 and A2 peptide units which remain linked by a disulfide bond
  • Once the A subunit is internalized by the eukaryotic cell, the disulfide bond is reduced
pathway continued
Pathway continued

The A1 subunit contains a ADP-ribosyltransferase which covalently modifies the G protein, which regulates adenylate cyclase. Adenylate cyclase mediates the formation of cAMP

The increase in cAMP levels bring about the secretion of chloride and bicarbonate from the mucosal cells into the intestinal lumen

The change in ion concentrations leads to the secretion of large amounts of water into the lumen, known as diarrhea

genetics of cholera toxin
Genetics of Cholera Toxin

Genes encoding CT

ctxAB - recognized to be the genome of a filamentous phage CTXΦ (ctxA and ctxB)

Transcription of ctxAB is regulated by several proteins

CTXΦ genome can integrate into the host genome at a specific site, attRS

The CTX genetic element also has a “core” region carrying several phage morphogenesis genes

These entire CTX gene set is flanked repeated sequences, the attRS1 site

The entire genetic element is 6.9kb

The receptor for CTXΦ is Toxin-Coregulated Pili (TCP)

toxin coregulated pili
Toxin-Coregulated Pili

Efficient colonization of V. cholera in the small intestine requires the expression of TCP’s

  • TCP’s are expressed on the surface of V. cholera
  • TCP’s are long laterally associated filaments

The major pilin subunit is TcpA

Genes for TCP production are clustered on the pathogenicity island located on chromosome 2

regulation of virulence factors
Regulation of Virulence Factors

Expression of CT & TCP have been shown in vitro to be strongly influenced by changes in cultural conditions

ie. temperature, pH, osmolarity, &

growth medium composition

CT & TCP are regulated via a cascade in

which ToxR and TcpP control expression

of ToxT, which is a transcriptional activator

directly controlling several virulence genes

ToxR & TcpP are inner-membrane proteins

which interact with other transmembrane

regulatory proteins

ToxR/S proteins are required for transcription of toxT gene and are also important for ctx transcription and regulation other outer membrane proteins

recap of phage movement
Recap of phage movement

V. cholerae did not always cause disease. Infection with the CTX phage gives the bacterium its toxinogenicity. The phage recognizes a pilus on the surface of the bacterium and uses it to enter the cell. Once inside the cell, the CTX phage integrates into the chromosome and the lysogen expresses cholera toxin.

The CTX phage has received special attention because it is the first filamentous phage found to transfer toxin genes to its host. The important lesson from this discovery is that many different types of phage may carry virulence factors, and transfer of virulence genes by phage may be a major mechanism of evolution of new bacterial diseases.